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Heterologous expression of an Agaricus meleagris pyranose dehydrogenase-encoding gene in Aspergillus spp. and characterization of the recombinant enzyme

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Abstract

Pyranose dehydrogenase (PDH) is a flavin-dependant sugar oxidoreductase found in the family Agaricaceae, basidiomycetes that degrade lignocellulose-rich forest litter, and is catalytically related to the fungal enzymes pyranose 2-oxidase and cellobiose dehydrogenase. It has broad substrate specificity and displays similar activities with most sugar constituents of lignocellulose including disaccharides and oligosaccharides, a number of (substituted) quinones, and metal ions are suitable electron acceptors rather than molecular oxygen. In contrast to pyranose 2-oxidase and cellobiose dehydrogenase, which oxidize regioselectively at C-2 and C-1, respectively, PDH is capable of oxidation on C-1 to C-4 as well as double oxidations, depending on the nature of the substrate. This makes it a very interesting enzyme for biocatalytic applications, as many of the reaction products are otherwise unaccessible by chemical or enzymatic means. PDH was characterized in detail in a limited number of fungi, and the first encoding genes were isolated only recently. We report here, for the first time, the heterologous expression of one of these genes, encoding the major PDH protein in Agaricus meleagris, in the filamentous fungi Aspergillus nidulans, and Aspergillus niger.

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Acknowledgments

This study was financed by grant L210-B11 to CP from the Translational Research Programme of the Austrian Science Foundation (FWF) and the Forschungsförderungsgesellschaft FFG (K+ Programme for the Research Centre for Applied Biocatalysis)

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Author notes

  1. Oliver Spadiut

    Present address: School of Biosciences, Royal Institute of Technology, Stockholm, Sweden

Authors and Affiliations

  1. Department of Food Sciences and Technology, University of Natural Resources and Applied Life Sciences (BOKU), Vienna, Austria

    Ines Pisanelli, Doris Gschnitzer, Oliver Spadiut & Clemens Peterbauer

  2. Research Centre Applied Biocatalysis, Graz, Austria

    Magdalena Kujawa

  3. Institute of Chemical Engineering, University of Technology, Vienna, Austria

    Bernhard Seiboth

  4. Department für Lebensmittelwissenschaften und-technologie, Universität für Bodenkultur, Muthgasse 18, 1190, Wien, Austria

    Clemens Peterbauer

Authors
  1. Ines Pisanelli
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  2. Magdalena Kujawa
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  3. Doris Gschnitzer
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  4. Oliver Spadiut
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  5. Bernhard Seiboth
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  6. Clemens Peterbauer
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Correspondence to Clemens Peterbauer.

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Pisanelli, I., Kujawa, M., Gschnitzer, D. et al. Heterologous expression of an Agaricus meleagris pyranose dehydrogenase-encoding gene in Aspergillus spp. and characterization of the recombinant enzyme. Appl Microbiol Biotechnol 86, 599–606 (2010). https://doi.org/10.1007/s00253-009-2308-x

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  • DOI: https://doi.org/10.1007/s00253-009-2308-x

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