Abstract
Nowadays, reports of antimicrobial resistance (AMR) against many antibiotics are increasing because of their misapplication. With this rise, there is a serious decrease in the discovery and development of new types of antibiotics amid an increase in multi-drug resistance. Unfermented Acinetobacter baumannii from gram-negative bacteria, which is one of the main causes of nosocomial infections and multi-drug resistance, has 4 main kinds of antibiotic resistance mechanism: inactivating antibiotics by enzymes, reduced numbers of porins and changing of their target or cellular functions due to mutations, and efflux pumps. In this study, characterization of the possible mutations in OprD (OccAB1) porins from hospital strains of A. baumannii were investigated using single channel electrophysiology and compared with the standard OprD isolated from wild type ATCC 19,606. For this aim, 5 A. baumannii bacteria samples were obtained from patients infected with A. baumannii, after which OprD porins were isolated from these A. baumannii strains. OprD porins were then inserted in an artificial lipid bilayer and the current–voltage curves were obtained using electrical recordings through a pair of Ag/AgCl electrodes. We observed that each porin has a characteristic conductance and single channel recording, which then leads to differences in channel diameter. Finally, the single channel data have been compared with the gene sequences of each porin. It was interesting to find out that each porin isolated has a unique porin diameter and decreased anion selectivity compared to the wild type.
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Acknowledgements
The authors would like to acknowledge funding from TUBITAK under grant number 117S114. The authors would also like to thank Prof. İ. Agah İnce, Dr. Araz N. Dizaji, Prof. Mathias Winterhalter and Dr. Daniel Pletzer for useful discussions in general and Prof. Barış Otlu for useful discussions during the selection of clinical samples.
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Ebrahimi, A., Ergün, T., Kaygusuz İzgördü, Ö. et al. Revealing the single-channel characteristics of OprD (OccAB1) porins from hospital strains of Acinetobacter baumannii. Eur Biophys J 52, 131–143 (2023). https://doi.org/10.1007/s00249-023-01651-2
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DOI: https://doi.org/10.1007/s00249-023-01651-2