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Dihydrolipoic acid reduces cytochrome b561 proteins

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Abstract

Cytochrome b561 (Cyt-b561) proteins constitute a family of trans-membrane proteins that are present in a wide variety of organisms. Two of their characteristic properties are the reducibility by ascorbate (ASC) and the presence of two distinct b-type hemes localized on two opposite sides of the membrane. Here we show that the tonoplast-localized and the putative tumor suppressor Cyt-b561 proteins can be reduced by other reductants than ASC and dithionite. A detailed spectral analysis of the ASC-dependent and dihydrolipoic acid (DHLA)-dependent reduction of these two Cyt-b561 proteins is also presented. Our results are discussed in relation to the known antioxidant capability of DHLA as well as its role in the regeneration of other antioxidant compounds of cells. These results allow us to speculate on new biological functions for the trans-membrane Cyt-b561 proteins.

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Acknowledgments

This work was supported by a grant from the Flemish Fund for Scientific Research (FWO; G.0118.07N) to H.A. and from the Hungary-Romania Cross Border Cooperation Program of the EU (HURO/0901/219) to A.B. and L.Z.

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Authors and Affiliations

  1. Biological Research Centre, Institute of Biophysics, Hungarian Academy of Sciences, Temesvári krt. 62, 6726, Szeged, Hungary

    Alajos Bérczi & László Zimányi

  2. Department of Biology, University of Antwerp, Campus Groenenborger, Groenenborgerlaan 171, 2020, Antwerpen, Belgium

    Han Asard

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  1. Alajos Bérczi
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  2. László Zimányi
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  3. Han Asard
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Correspondence to Alajos Bérczi.

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Special Issue: Structure, function, folding and assembly of membrane proteins—Insight from Biophysics.

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Bérczi, A., Zimányi, L. & Asard, H. Dihydrolipoic acid reduces cytochrome b561 proteins. Eur Biophys J 42, 159–168 (2013). https://doi.org/10.1007/s00249-012-0812-x

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  • DOI: https://doi.org/10.1007/s00249-012-0812-x

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