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Transverse and tangential orientation of predicted transmembrane fragments 4 and 10 from the human multidrug resistance protein (hMRP1/ABCC1) in membrane mimics

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Abstract

The human multidrug-resistance-associated protein 1 (hMRP1/ABCC1) belongs to the large ATP-binding cassette transporter superfamily. In normal tissues, hMRP1 is involved in tissue defense, whereas, in cancer cells, it is overproduced and contributes to resistance to chemotherapy. We previously investigated the folding properties of the predicted transmembrane fragments (TM) TM16, and TM17 from membrane-spanning domain 2 (MSD2). These TMs folded only partially as an α-helix and were located in the polar headgroup region of detergent micelles used as membrane mimics (Vincent et al. in Biochim Biophys Acta 1768:538–552, 2007; de Foresta et al. in Biochim Biophys Acta 1798:401–414, 2010). We have now extended these studies to TM4 and TM10, from MSD0 and MSD1, respectively. TM10 may be involved in the substrate translocation pathway whereas the role of TM4 is less predictable, because few studies have focused on MSD0, a domain present in some hMRP1 homologs only. Each TM contained a single Trp residue (W142 or W553) acting as an intrinsic fluorescent probe. The location and dynamics of the TMs in dodecylphosphocholine (DPC) or n-dodecyl-β-d-maltoside (DDM) micelles were studied by Trp steady-state and time-resolved fluorescence, including quenching experiments. Overall TM structure was analyzed by far-UV circular dichroism studies in detergent micelles and TFE. TM10 behaved similarly to TM16 and TM17, with an interfacial location in micelles consistent with a possible role in lining the transport pore. By contrast, TM4 behaved like a classical TM fragment with a high α-helical content, and its transmembrane insertion did not require its interaction with other TMs.

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Abbreviations

hMRP1 (or ABCC1):

Human multidrug-resistance protein 1

BCRP (or ABCG2):

Breast cancer-resistance protein

LTC4 :

Cysteinyl leukotriene C4

E217βG:

Estradiol 17-(β-d-glucuronide)

GSH:

Reduced glutathione

DDM:

n-dodecyl-β-d-maltoside

BrDDM (BrDM in our previous papers):

7,8-Dibromododecylmaltoside

BrUM:

10,11-Dibromoundecanoylmaltoside

DPC:

Dodecylphosphocholine

Cmc:

Critical micellar concentration

NATA:

N-acetyltryptophanamide

TOE:

Tryptophan octyl ester

DMSO:

Dimethylsulfoxide

TFE:

Trifluoroethanol

TFA:

Trifluoroacetic acid

MSD:

Membrane-spanning domain

TM:

Transmembrane fragment

NBD:

Nucleotide-binding domain

MEM:

Maximum entropy method

CD:

Circular dichroism

FWHM:

Full width at half maximum

P3:

K2WL9AL9K2A

P5:

K2CLWL7AL9K2A

P7:

K2CL3WL5AL9K2A

P9:

K2CL5WL3AL9K2A

P11:

K2CL7WLAL9K2A

P13:

K2CL9WL9K2A

TM16:

A1195NRWLAVRLECVGNCIVLFAALFAV1219

mTM16:

A1195NRWLAVRLESVGNSIVLFAALFAV1219

TM17:

A1227GLVGLSVSYSLQVTTYLNWLVRMS1251

mTM17:

K1227GLVGLSVSYSLQVTTYLNWLVRMS1251

TM4:

V132QSSGIMLTFWLVALVCALAILRSK156

mTM4:

V132QSSGIMLTFWLVALVSALAILRSK156

TM10:

S546AVGTFTWVCTPFLVALCTFAVYVT570

mTM10:

S546AVGTFTWVSTPFLVALSTFAVYVT570

(all synthetic peptides were Ac– and –Am)

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Authors and Affiliations

  1. CEA, iBiTecS, Service de Bioénergétique Biologie Structurale et Mécanismes, 91191, Gif-sur-Yvette, France

    Béatrice de Foresta & Manuel Garrigos

  2. CNRS, URA 2096, 91191, Gif-sur-Yvette, France

    Béatrice de Foresta & Manuel Garrigos

  3. Institut de Biochimie et Biophysique Moléculaire et Cellulaire, Université Paris-Sud 11, UMR 8619, Bât. 430, Orsay, France

    Michel Vincent & Jacques Gallay

  4. CNRS, UMR 8619, Orsay, France

    Michel Vincent & Jacques Gallay

Authors
  1. Béatrice de Foresta
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  2. Michel Vincent
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  3. Manuel Garrigos
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  4. Jacques Gallay
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Corresponding authors

Correspondence to Béatrice de Foresta or Jacques Gallay.

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de Foresta, B., Vincent, M., Garrigos, M. et al. Transverse and tangential orientation of predicted transmembrane fragments 4 and 10 from the human multidrug resistance protein (hMRP1/ABCC1) in membrane mimics. Eur Biophys J 40, 1043–1060 (2011). https://doi.org/10.1007/s00249-011-0721-4

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  • DOI: https://doi.org/10.1007/s00249-011-0721-4

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