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QCM-D fingerprinting of membrane-active peptides

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Abstract

The increasing prevalence of antibiotic-resistant bacteria is becoming a public health crisis. Antimicrobial peptides (AMPs) are a promising solution, because bacterial resistance is less likely. Quartz crystal microbalance with dissipation monitoring (QCM-D) is a versatile and valuable technique for investigation of these peptides. This article looks at the different approaches to the interpretation of QCM-D data, showing how to extract the maximum information from the data. Five AMPs of diverse charge, length and activity are used as case studies: caerin 1.1 wild-type, two caerin 1.1 mutants (Gly15Gly19-caerin 1.1 and Ala15Ala19-caerin 1.1), aurein 1.2 and oncocin. The interaction between the AMP and a 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membrane is analysed inter alia using frequency–dissipation plots (∆f–∆D plots) to ascertain the mechanism of action of the AMP. The ∆f–∆D plot can then be used to provide a fingerprint for the AMP–membrane interaction. Building up a database of these fingerprints for all known AMPs will enable the relationship between AMP structure and membrane activity to be better understood, hopefully leading to the future development of antibiotics without bacterial resistance.

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Abbreviations

AMP:

Antimicrobial peptide

QCM-D:

Quartz crystal microbalance with dissipation monitoring

DMPC:

1,2-Dimyristoyl-sn-glycero-3-phosphocholine

MPA:

3-Mercaptopropionic acid

DMPG:

1,2-Dimyristoyl-sn-glycero-3-phospho-rac-(1-glycerol)

PBS:

Phosphate buffered saline

AFM:

Atomic force microscopy

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Authors and Affiliations

  1. School of Chemistry, Monash University, Clayton, VIC, 3800, Australia

    George A. McCubbin, Slavica Praporski, Stefania Piantavigna & Lisandra L. Martin

  2. Institute of Bioanalytical Chemistry, Leipzig University, 04103, Leipzig, Germany

    Daniel Knappe & Ralf Hoffmann

  3. Department of Chemistry, The University of Adelaide, Adelaide, SA, 5005, Australia

    John H. Bowie

  4. School of Chemistry, Bio21 Institute, University of Melbourne, Melbourne, VIC, 3010, Australia

    Frances Separovic

Authors
  1. George A. McCubbin
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  2. Slavica Praporski
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  3. Stefania Piantavigna
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  4. Daniel Knappe
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  5. Ralf Hoffmann
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  6. John H. Bowie
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  7. Frances Separovic
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  8. Lisandra L. Martin
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Corresponding author

Correspondence to Lisandra L. Martin.

Additional information

Membrane-active peptides: 455th WE-Heraeus-Seminar and AMP 2010 Workshop.

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McCubbin, G.A., Praporski, S., Piantavigna, S. et al. QCM-D fingerprinting of membrane-active peptides. Eur Biophys J 40, 437–446 (2011). https://doi.org/10.1007/s00249-010-0652-5

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  • DOI: https://doi.org/10.1007/s00249-010-0652-5

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