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Quantification of the effect of glycocalyx condition on membrane receptor interactions using an acoustic wave sensor

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Abstract

The effect of the cell glycocalyx on the binding of a membrane receptor, class I major histocompatibility complex (MHC) human leukocyte antigen (HLA)-A2, to an immobilized anti-HLA antibody was investigated using an acoustic sensor based on a Love wave geometry. The enzyme neuraminidase was used to remove sialic acid residues from the cell glycocalyx. Real-time measurements of the amplitude of the acoustic wave showed that treatment with neuraminidase facilitates HLA/anti-HLA-mediated cell attachment via a 3.6-fold increase of the two-dimensional (2D) binding constant of the interaction. This could be attributed to better approach of binding partners due to favorable condition of the desialylated glycocalyx. The results underline the importance of microtopological factors in membrane receptor binding and reveal the potential of the Love wave sensor and 2D binding parameters for studying cell–substrate binding events.

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Notes

  1. The 2D kinetics and affinity of the HLA/anti-HLA interaction for untreated LG2 cells had previously been calculated as k a = 1.15 × 10−5 μm2 s−1 per molecule, k d = 2.07 × 10−5 s−1, and K A = 0.556 μm2 per molecule (Saitakis et al. 2008). In that work, the kinetic analysis was performed for real-time acoustic data up to the point of buffer exchange. In the current study, we included data up to the point of signal equilibrium, since there was still HLA/anti-HLA binding taking place on the sensor surface. We believe that this modification is justified, since it includes the whole real-time data for the association reaction.

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Acknowledgments

The authors would like to acknowledge the financial support of ELKE, University of Crete (research grant K. A. 2732) and the General Secretariat of Research and Technology (PENED 03ED623).

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Authors and Affiliations

  1. Institute of Molecular Biology and Biotechnology, FO.R.T.H, 71110, Heraklion, Greece

    Michael Saitakis & Electra Gizeli

  2. Department of Biology, University of Crete, 71409, Heraklion, Greece

    Michael Saitakis & Electra Gizeli

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  1. Michael Saitakis
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  2. Electra Gizeli
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Correspondence to Electra Gizeli.

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Saitakis, M., Gizeli, E. Quantification of the effect of glycocalyx condition on membrane receptor interactions using an acoustic wave sensor. Eur Biophys J 40, 209–215 (2011). https://doi.org/10.1007/s00249-010-0632-9

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  • DOI: https://doi.org/10.1007/s00249-010-0632-9

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