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Structural themes and variations in protein kinase A as seen by small-angle scattering and neutron contrast variation

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Abstract

Using small-angle solution scattering and neutron contrast variation, we have studied the structure of the multi-subunit protein kinase A. We have gained insights into how nature can take a set of common structural domains (or themes) and modulate their interactions via sequence variations and second messenger mediated signaling to affect enzyme activity and receptor binding important for targeting this multi-function enzyme to specific sub-cellular locations. These studies demonstrate the power of neutron contrast variation to expand our knowledge of the dynamic supra-molecular structures that carry out biological function.

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Acknowledgements

The work summarized in this review is the result of a number of collaborations including Donald K. Blumenthal, Sharron Boylan, Simon Brown, William Heller, Elaine Hoye, Ryan Mitchell, Glenn Olah, Tobin Sosnick, Susan Taylor, Chang-Shung Tung, Dominico Vigil, Donal Walsh, and Jinkui Zhao.

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  1. School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia

    Jill Trewhella

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  1. Jill Trewhella
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Correspondence to Jill Trewhella.

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Trewhella, J. Structural themes and variations in protein kinase A as seen by small-angle scattering and neutron contrast variation. Eur Biophys J 35, 585–589 (2006). https://doi.org/10.1007/s00249-006-0061-y

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  • DOI: https://doi.org/10.1007/s00249-006-0061-y

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