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Effects of Reductive Soil Disinfestation Combined with Liquid-Readily Decomposable Compounds and Solid Plant Residues on the Bacterial Community and Functional Composition

  • Soil Microbiology
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Abstract

Reductive soil disinfestation (RSD) incorporated with sole plant residues or liquid-readily decomposable compounds is an effective management strategy to improve soil health. However, the synthetic effects of RSD incorporated with liquid-readily decomposable compounds and solid plant residues on soil ecosystem services remain unclear. Field experiments were carried out to investigate the effects of untreated soil (CK), RSD incorporated with sawdust (SA), molasses (MO), and their combinations (SA + MO) on the bacterial community and functional composition. The results showed that RSD treatments significantly altered soil bacterial community structure compared to CK treatment. The bacterial community structure and composition in MO and SA + MO treatments were clustered compared to SA treatment. This was mainly attributed to the readily decomposable carbon sources in molasses having a stronger driving force to reshape the soil microbial community during the RSD process. Furthermore, the functional compositions, such as the disinfestation efficiency of F. oxysporum (96.4 − 99.1%), abundances of nitrogen functional genes, soil metabolic activity, and functional diversity, were significantly increased in all of the RSD treatments. The highest disinfestation efficiency and abundances of denitrification (nirS and nrfA) and nitrogen fixation (nifH) genes were observed in SA + MO treatment. Specifically, SA + MO treatment enriched more abundant beneficial genera, e.g., Oxobacter, Paenibacillus, Cohnella, Rummeliibacillus, and Streptomyces, which were significantly and positively linked to disinfestation efficiency, soil metabolic activity, and denitrification processes. Our results indicated that combining RSD practices with liquid-readily decomposable compounds and solid plant residues could effectively improve soil microbial community and functional composition.

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Data Availability

The raw sequencing data were deposited at the NCBI Sequence Read Archive database with the accession number of PRJNA858391.

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Acknowledgements

We thank Yonggen Yuan for providing the experimental field of this study. The authors also thank the editors and reviewers for their valuable comments and suggestions.

Funding

This work was financially supported by the National Natural Science Foundation of China (Grant No. 32160748 and 42090065), the Key Research and Development Project (Agriculture) of Yichun City, Jiangxi Province (20211YFN4240), and the China Postdoctoral Science Foundation (2021M691625).

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  1. Yuanyuan Yan and Yi Xie contributed equally to this work.

Authors and Affiliations

  1. Engineering Technology Research Center of Jiangxi Universities and Colleges for Selenium Agriculture, College of Life Science and Environmental Resources, Yichun University, Yichun, 336000, China

    Yuanyuan Yan, Yi Xie & Liangliang Liu

  2. School of Geography, Nanjing Normal University, Nanjing, 210023, China

    Yuanyuan Yan, Yi Xie, Jingqing Zhang, Ruimin Li, Ahmad Ali, Zucong Cai, Xinqi Huang & Liangliang Liu

  3. Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing, 210023, China

    Zucong Cai & Xinqi Huang

  4. Jiangsu Engineering Research Center for Soil Utilization & Sustainable Agriculture, Nanjing Normal University, Nanjing, 210023, China

    Zucong Cai & Xinqi Huang

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Zucong Cai, Xinqi Huang, and Liangliang Liu conceived designed research. Yuanyuan Yan, Yi Xie, Jingqing Zhang, and Ruimin Li performed the experiment and collected the data. Yuanyuan Yan, Liangliang Liu, and Ahmad Ali analyzed the data. Yuanyuan Yan and Yi Xie wrote and revised the manuscript. All authors read and approved the manuscript.

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Yan, Y., Xie, Y., Zhang, J. et al. Effects of Reductive Soil Disinfestation Combined with Liquid-Readily Decomposable Compounds and Solid Plant Residues on the Bacterial Community and Functional Composition. Microb Ecol 86, 1132–1144 (2023). https://doi.org/10.1007/s00248-022-02139-w

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