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Single Cell Analysis Reveals a New Microsporidia-Host Association in a Freshwater Lake

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Abstract

Microsporidia are a large group of obligate intracellular eukaryotic parasites. Recent studies suggest that their diversity can be huge in freshwater lake ecosystems especially in the < 150-µm size fraction. However, little is known about their hosts and therefore their impact on the trophic food web functioning. In this study, single cell analysis and fluorescence microscopy were used to detect new host-parasite association within rotifer communities in lake Aydat (France). Our analysis showed the existence of a potential new species belonging to the Crispospora genus able of infecting the rotifer Kellicottia with a high prevalence (42.5%) suggesting that Microsporidia could have a great impact on the rotifer populations’ regulation in lakes.

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Data Availability

All data and material are available in this publication and additional information online (supplementary material and supplementary figures). Sequences are available on GenBank, accession numbers from ON026091 to ON026096 for the microsporidian sequences and from ON033621 to ON033624 for the rotifers.

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Acknowledgements

The authors are very grateful to Jonathan Colombet and Eugénie Carriere for their help with sampling. We also gratefully acknowledge the platform CLIC (CLermont-ferrand Imagerie Confocale, Université Clermont Auvergne) and especially Caroline Vachias for her assistance with the confocal microscopy. The work of MC and AM was supported by two PhD fellowships funded by the ‘Ministère de l’Enseignement Supérieur de la Recherche et de l’Innovation’.

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  1. Marina Chauvet and Arthur Monjot contributed equally to this work

Authors and Affiliations

  1. CNRS, Laboratoire Microorganismes : Génome Et Environnement, Université Clermont Auvergne, 63000, Clermont-Ferrand, France

    Marina Chauvet, Arthur Monjot, Anne Moné & Cécile Lepère

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  1. Marina Chauvet
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  2. Arthur Monjot
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  3. Anne Moné
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  4. Cécile Lepère
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Correspondence to Cécile Lepère.

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The authors declare no competing interests.

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Chauvet, M., Monjot, A., Moné, A. et al. Single Cell Analysis Reveals a New Microsporidia-Host Association in a Freshwater Lake. Microb Ecol 85, 1630–1633 (2023). https://doi.org/10.1007/s00248-022-02023-7

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