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Distribution of Viruses Inhabiting Heterobasidion annosum in a Pine-Dominated Forest Plot in Southern Finland

  • Fungal Microbiology
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An Erratum to this article was published on 18 September 2017

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Abstract

We investigated the diversity and spatial distribution of viruses infecting strains of the root rot fungus Heterobasidion annosum collected from pine stumps at a heavily infected forest site. Four different partitiviruses were detected in 14 H. annosum isolates at the study site, constituting approximately 29% of all Heterobasidion isolates investigated (N = 48). Two of the viruses detected were new partitiviruses designated here as Heterobasidion partitivirus 16 (HetPV16) and HetPV20, and two were previously known partitiviruses: HetPV7 and HetPV13. The two new partitiviruses found, HetPV16-an1 and HetPV20-an1, shared ~70% RdRp nucleotide sequence identity with the alphapartitivirus Rosellinia necatrix partitivirus 2, and less than 40% identity with known viruses of Heterobasidion spp. HetPV7-an1 was closely similar to HetPV7-pa1 isolated earlier from Heterobasidion parviporum, supporting the view of conspecific virus pools in different Heterobasidion species. Three fungal isolates were found to be co-infected with two different partitivirus strains (HetPV7-an1 and HetPV13-an2 or HetPV16-an1 and HetPV20-an1). Different isolates representing each host clone had variable virus compositions, and virus strains occurring in more than one host clone showed minor sequence variations between clones.

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  • 18 September 2017

    An erratum to this article has been published.

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Acknowledgments

We are grateful to Juha Puranen, Marja-Leena Santanen, Ari Rajala, Minna Oksanen, Sonja Sarsila, Päivi Räisänen, and Belen Espinoza for technical assistance.

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Authors and Affiliations

  1. Natural Resources Institute Finland, Latokartanonkaari 9, 00790, Helsinki, Finland

    Rafiqul Hyder, Tuula Piri, Jarkko Hantula, Heikki Nuorteva & Eeva J. Vainio

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  1. Rafiqul Hyder
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  2. Tuula Piri
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  3. Jarkko Hantula
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  4. Heikki Nuorteva
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  5. Eeva J. Vainio
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Correspondence to Rafiqul Hyder.

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Funding

The study was funded by the Academy of Finland (decision numbers 251193 and 258520).

Conflict of Interest

The authors declare that they have no conflict of interest.

Additional information

The nucleotide sequence data reported are available in the GenBank databases under the accession numbers: KY859973-KY859980 and KY911256-KY911275.

An erratum to this article is available at https://doi.org/10.1007/s00248-017-1067-y.

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Hyder, R., Piri, T., Hantula, J. et al. Distribution of Viruses Inhabiting Heterobasidion annosum in a Pine-Dominated Forest Plot in Southern Finland. Microb Ecol 75, 622–630 (2018). https://doi.org/10.1007/s00248-017-1027-6

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