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Microbial Ecology

, Volume 73, Issue 2, pp 466–478 | Cite as

Comparative Assessment of Mediterranean Gorgonian-Associated Microbial Communities Reveals Conserved Core and Locally Variant Bacteria

  • Jeroen A. J. M. van de Water
  • Rémy Melkonian
  • Christian R. Voolstra
  • Howard Junca
  • Eric Beraud
  • Denis Allemand
  • Christine Ferrier-Pagès
Host Microbe Interactions

Abstract

Gorgonians are key habitat-forming species of Mediterranean benthic communities, but their populations have suffered from mass mortality events linked to high summer seawater temperatures and microbial disease. However, our knowledge on the diversity, dynamics and function of gorgonian-associated microbial communities is limited. Here, we analysed the spatial variability of the microbiomes of five sympatric gorgonian species (Eunicella singularis, Eunicella cavolini, Eunicella verrucosa, Leptogorgia sarmentosa and Paramuricea clavata), collected from the Mediterranean Sea over a scale of ∼1100 km, using next-generation amplicon sequencing of the 16S rRNA gene. The microbiomes of all gorgonian species were generally dominated by members of the genus Endozoicomonas, which were at very low abundance in the surrounding seawater. Although the composition of the core microbiome (operational taxonomic units consistently present in a species) was found to be unique for each host species, significant overlap was observed. These spatially consistent associations between gorgonians and their core bacteria suggest intricate symbiotic relationships and regulation of the microbiome composition by the host. At the same time, local variations in microbiome composition were observed. Functional predictive profiling indicated that these differences could be attributed to seawater pollution. Taken together, our data indicate that gorgonian-associated microbiomes are composed of spatially conserved bacteria (core microbiome members) and locally variant members, and that local pollution may influence these local associations, potentially impacting gorgonian health.

Keywords

Endozoicomonas Gorgonian Coral 16S rRNA gene Bacterial community Holobiont Evolution 

Notes

Acknowledgments

The authors would like to thank Stéphanie Reynaud and Cécile Rottier (Scientific Centre of Monaco), Covadonga Orejas Saco del Valle (Spanish Institute of Oceanography, Majorca), Petar Kružić (University of Zagreb, Croatia), Joseph Gili (Marine Science Institute, Spain) and Giorgio Bavestrello (University of Genova, Italy) for their help with the sample collections and Ursula Pilat for the sample processing. Lauren Yum, the Bioscience Core Lab from King Abdullah University of Science and Technology (KAUST), and Till Bayer are thanked for their assistance with the 454 amplicon library preparation, sequencing and preliminary analysis, respectively. We would also like to thank the Paul Hamel Foundation and KAUST for the financial support.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Jeroen A. J. M. van de Water
    • 1
  • Rémy Melkonian
    • 1
  • Christian R. Voolstra
    • 2
  • Howard Junca
    • 3
  • Eric Beraud
    • 1
  • Denis Allemand
    • 1
  • Christine Ferrier-Pagès
    • 1
  1. 1.Centre Scientifique de MonacoMonacoMonaco
  2. 2.Red Sea Research CenterKing Abdullah University of Science and Technology (KAUST)ThuwalSaudi Arabia
  3. 3.Microbiomas Foundation - Division of Ecogenomics & HolobiontsChiaColombia

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