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Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China

  • Environmental Microbiology
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Abstract

Mariculture is one of the major seafood supplies worldwide and has caused serious environmental concerns on the coastal zone. Its rapid development has been shown to disrupt the sediment ecosystems and thus influence the benthic bacterial communities. Bacterial diversity and community structure within both adjacent farms and non-cultured zones intertidal sediments along the coasts of Qinhuangdao and Dalian, China, were investigated using full-length 16S rRNA gene-based T-RFLP analyses and clone library construction. Richness and Shannon–Wiener index were significantly increased at sites adjacent the mariculture farm with mean values of 29 and 2.97 from peak profiles of T-RFLP result. Clustering analyses suggested that impacts of mariculture on bacterial diversity of sediment were significantly larger than those resulted from temporal and spatial scales. Upon comparisons of RFLP patterns from 602 clones from libraries of the selected five samples, 137 OTUs were retrieved. Members of γ- and δ-Proteobacteria, Bacilli, Flavobacteria, and Actinobacteria were recorded in all libraries. In addition, γ-Proteobacteria were dominant in all samples (21.7~45.0 %). Redundancy analysis revealed that the distribution of bacterial composition seemed to be determined by the variables of salinity, PO4 3−-P, NH4 +-N, and Chlorophyll a content. The phyla of γ-Proteobacteria, Clostridia, Flavobacteria, Bacilli, and Planctomycetes were principal components to contribute to the bacterial differences of clone libraries. Our finding demonstrated that these phyla could display variations of bacterial composition linked to environmental disturbance resulted from mariculture.

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Acknowledgments

The authors would like to thank Zhonghua Wang, Lixue Wen, and Chunjuan Yuan for their technical assistance. This research work was financially supported by grants from the National Natural Science Foundation of China 41106100 and the CAS/SAFEA International Partnership Program for Creative Research Teams “Representative environmental processes and resources effects in coastal zone.”

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Authors and Affiliations

  1. Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China

    Jialin Li, Shuxian Yu & Song Qin

  2. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Fuchao Li

  3. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Shuxian Yu

  4. School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China

    Guangyi Wang

  5. Department of Microbiology, University of Hawaii at Manoa, Honolulu, HI, 96822, USA

    Guangyi Wang

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  1. Jialin Li
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Correspondence to Song Qin or Guangyi Wang.

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Li, J., Li, F., Yu, S. et al. Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China. Microb Ecol 66, 861–870 (2013). https://doi.org/10.1007/s00248-013-0272-6

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