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Effect of Dietary Prebiotic (Mannan Oligosaccharide) Supplementation on the Caecal Bacterial Community Structure of Turkeys

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Abstract

The identification of specific bacterial species influenced by mannan oligosaccharide (MOS) supplementation may assist in the formulation of new and improved diets that promote intestinal health and improve bird performance, offering suitable alternatives to antimicrobials in feed for sustainable poultry production. This study has been conducted to evaluate the use of a MOS compound derived from the yeast cell wall of Saccharomyces cerevisiae on turkey performance, bacterial community structure and their phylogenetic associations. A 42-day turkey trial was carried out on birds fed control and MOS-supplemented diets. Bird performance data (weight gains, feed consumption and feed efficiency ratios) were collected, and caecal contents were extracted from randomly caught poults on days 28, 35 and 42 posthatch. Bird performance data showed no improvements as a result of dietary supplementation. Automated ribosomal intergenic spacer analysis (ARISA) revealed the bacterial community structure to be significantly altered on days 28 and 35 posthatch but not day 42 as a result of dietary supplementation. This technique was coupled with 16S rRNA gene sequence analysis to elucidate phylogenetic identities of bacteria. The dominant bacteria of the caecum on all days in both treatment groups were members of phylum Firmicutes, followed by the Bacteroidetes and Proteobacteria phyla, respectively. Statistical analysis of the 16S rRNA gene libraries showed that the composition of the MOS clone library differed significantly to the control on day 35 posthatch. It can be concluded that MOS alters the bacterial community structure in the turkey caecum.

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Bibliography

  1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    PubMed  CAS  Google Scholar 

  2. Anderson MJ (2001) Permutation tests for univariate or multivariate analysis of variance and regression. Can J Fish Aquat Sci 58:626–639

    Article  Google Scholar 

  3. Anderson MJ, Robinson J (2003) Generalised discriminant analysis based on distances. Aust N Z J Stat 45:301–318

    Article  Google Scholar 

  4. Barton MD (2000) Antibiotic use in animal feed and its impact on human health. Nutr Res Rev 13:279–299

    Article  PubMed  CAS  Google Scholar 

  5. Baurhoo B, Letellier A, Zhao X, Ruiz-Feria CA (2007) Cecal populations of Lactobacilli and Bifidobacteria and Escherichia coli populations after in vivo Escherichia coli challenge in birds fed diets with purified lignin or mannan oligosaccharides. Poult Sci 86:2509–2516

    Article  PubMed  CAS  Google Scholar 

  6. Bray JR, Curtis JT (1957) An ordination of the upland forest communities of Southern Wisconsin. Ecol Monogr 27:326–349

    Article  Google Scholar 

  7. Brown MV, Schwalbach MS, Hewson I, Fuhrman JA (2005) Coupling 16S-ITS rDNA clone libraries and automated ribosomal intergenic spacer analysis to show marine microbial diversity: development and application to a time series. Environ Microbiol 7:1466–1479

    Article  PubMed  CAS  Google Scholar 

  8. Castanon JIR (2007) History of the use of antibiotic as growth promoters in European poultry feeds. Poult Sci 86:2466–2471

    Article  PubMed  CAS  Google Scholar 

  9. Çetin N, Güçlü BK, Çetin E (2005) The effects of probiotic and mannan oligosaccharide on some haematological and immunological parameters in turkeys. J Vet Med Ser A 52:263–267

    Article  Google Scholar 

  10. Clarke KR (1993) Non-parametric multivariate analyses of changes in community structure. Aust Ecol 18:117–143

    Article  Google Scholar 

  11. Coates ME, Davies MK, Kon SK (1955) The effect of antibiotics on the intestine of the chick. Br J Nutr 9:110–119

    Article  PubMed  CAS  Google Scholar 

  12. Cornfield J, Tukey JW (1956) Average values of mean squares in factorials. Ann Math Stat 27:907–949

    Article  Google Scholar 

  13. Corrigan, A., K. Horgan, N. Clipson, and R. A. Murphy. 2011. The effect of dietary supplementation with a yeast mannan oligosaccharide on the bacterial community structure of broiler caecal contents. Appl Environ Microbiol.

  14. Dibner JJ, Richards JD (2005) Antibiotic growth promoters in agriculture: history and mode of action. Poult Sci 84:634–643

    PubMed  CAS  Google Scholar 

  15. Dicksved J, Halfvarson J, Rosenquist M, Jarnerot G, Tysk C, Apajalahti J, Engstrand L, Jansson JK (2008) Molecular analysis of the gut microbiota of identical twins with Crohn's disease. ISME J 2:716–727

    Article  PubMed  CAS  Google Scholar 

  16. Dumonceaux TJ, Hill JE, Hemmingsen SM, Van Kessel AG (2006) Characterization of intestinal microbiota and response to dietary virginiamycin supplementation in the broiler chicken. Appl Environ Microbiol 72:2815–2823

    Article  PubMed  CAS  Google Scholar 

  17. Fairchild AS, Grimes JL, Jones FT, Wineland MJ, Edens FW, Sefton AE (2001) Effects of hen age, Bio-Mos and Flavomycin on poult susceptibility to oral Escherichia coli challenge. Poult Sci 80:562–571

    PubMed  CAS  Google Scholar 

  18. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  19. Firon N, Ofek I, Sharon N (1983) Carbohydrate specificity of the surface lectins of Escherichia coli, Klebsiella pneumoniae, and Salmonella typhimurium. Carbohydr Res 120:235–249

    Article  PubMed  CAS  Google Scholar 

  20. Geier MS, Torok VA, Allison GE, Ophel-Keller K, Hughes RJ (2009) Indigestible carbohydrates alter the intestinal microbiota but do not influence the performance of broiler chickens. J Appl Microbiol 106:1540–1548

    Article  PubMed  CAS  Google Scholar 

  21. Gong J, Si W, Forster RJ, Huang R, Yu H, Yin Y, Yang C, Han Y (2007) 16S rRNA gene-based analysis of mucosa-associated bacterial community and phylogeny in the chicken gastrointestinal tracts: from crops to ceca. FEMS Microbiol Ecol 59:147–157

    Article  PubMed  CAS  Google Scholar 

  22. Hill JE, Hemmingsen SM, Goldade BG, Dumonceaux TJ, Klassen J, Zijlstra RT, Goh SH, Van Kessel AG (2005) Comparison of ileum microflora of pigs fed corn-, wheat-, or barley-based diets by chaperonin-60 sequencing and quantitative PCR. Appl Environ Microbiol 71:867–875

    Article  PubMed  CAS  Google Scholar 

  23. Hooge DM (2004) Turkey pen trials with dietary mannan oligosaccharides: meta-analysis, 1993-2003. Int J Poult Sci 3:179–188

    Article  Google Scholar 

  24. Hooge DM, Sims MD, Sefton AE, Connolly A, Spring P (2003) Effect of dietary mannan oligosaccharide, with or without bacitracin or virginiamycin, on live performance of broiler chickens at relatively high stocking density on new litter. J App Poult Res 12:461–467

    CAS  Google Scholar 

  25. Scupham AJ, Patton T, Bent E, Bayles D (2008) Comparison of the cecal microbiota of domestic and wild turkeys. Microb Ecol 56:322–331

    Article  PubMed  Google Scholar 

  26. Jukes TH, Cantor CR (1969) Evolution of protein molecules. In: Munro HN (ed) Mammalian protein metabolism., pp 21–132

    Google Scholar 

  27. Juskiewicz J, Zdunczyk Z, Jankowski J (2006) Growth performance and metabolic response of the gastrointestinal tract of turkeys to diets with different levels of mannan-oligosaccharide. World's Poult Sci J 62:612–625

    Google Scholar 

  28. Kiyoshi T, Roustam IA, Takafumi N, Koretsugu O, Mutsumi N, Hiroki M, Yoshimi B (1999) Rumen bacterial diversity as determined by sequence analysis of 16S rDNA libraries. FEMS Microbiol Ecol 29:159–169

    Article  Google Scholar 

  29. Kruskal J (1964) Multidimensional scaling by optimizing goodness of fit to a nonmetric hypothesis. Psychometrika 29:1–27

    Article  Google Scholar 

  30. Lane DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt EE, Goodfellow M (eds) Nucleic acid techniques in bacterial systematics. John Wiley, Chichester, pp 115–175

    Google Scholar 

  31. Lu J, Domingo J (2008) Turkey fecal microbial community structure and functional gene diversity revealed by 16S rRNA gene and metagenomic sequences. J Microbiol 46:469–477

    Article  PubMed  CAS  Google Scholar 

  32. Lu J, Idris U, Harmon B, Hofacre C, Maurer JJ, Lee MD (2003) Diversity and succession of the intestinal bacterial community of the maturing broiler chicken. Appl Environ Microbiol 69:6816–6824

    Article  PubMed  CAS  Google Scholar 

  33. Lu J, Santo Domingo JW, Hill S, Edge TA (2009) Microbial diversity and host-specific sequences of Canada goose feces. Appl Environ Microbiol 75:5919–5926

    Article  PubMed  CAS  Google Scholar 

  34. Lu J, Santo Domingo JW, Lamendella R, Edge T, Hill S (2008) Phylogenetic diversity and molecular detection of bacteria in gull feces. Appl Environ Microbiol 74:3969–3976

    Article  PubMed  CAS  Google Scholar 

  35. Matsui H, Kato Y, Chikaraishi T, Moritani M, Ban-Tokuda T, Wakita M (2009) Microbial diversity in ostrich ceca as revealed by 16S ribosomal RNA gene clone library and detection of novel Fibrobacter species. Anaerobe 16:83–93

    Article  PubMed  Google Scholar 

  36. Newman, K. 1994. Mannan-oligosaccharides: natural polymers with significant impact on the gastrointestinal microflora and the immune system, p. 167-174. In T. P. Lyons and K. A. Jacques. (ed.), Biotechnology in the feed industry: Proceedings of Alltech's Tenth Annual Symposium. Nottingham University Press

  37. Parks CW, Grimes JL, Ferket PR, Fairchild AS (2001) The effect of mannan oligosaccharides, bambermycins, and virginiamycin on performance of large white male market turkeys. Poult Sci 80:718–723

    PubMed  CAS  Google Scholar 

  38. Parks CW, Grimes JL, Ferkett PR (2005) Effects of virginiamycin and a mannan oligosaccharide-virginiamycin shuttle program on the growth and performance of large white female turkeys. Poult Sci 84:1967–1973

    PubMed  CAS  Google Scholar 

  39. Phillips, M. L. 2009. Gut reaction: environmental effects on the human microbiota. Environ Health Perspect 117

  40. Ranjard L, Poly F, Lata JC, Mougel C, Thioulouse J, Nazaret S (2001) Characterization of bacterial and fungal soil communities by automated ribosomal intergenic spacer analysis fingerprints: biological and methodological variability. Appl Environ Microbiol 67:4479–4487

    Article  PubMed  CAS  Google Scholar 

  41. Roberfroid M (2007) Prebiotics: the concept revisited. J Nutr 137:830S–837S

    PubMed  CAS  Google Scholar 

  42. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  43. Salzman NH, de Jong H, Paterson Y, Harmsen HJM, Welling GW, Bos NA (2002) Analysis of 16S libraries of mouse gastrointestinal microflora reveals a large new group of mouse intestinal bacteria. Microbiology 148:3651–3660

    PubMed  CAS  Google Scholar 

  44. Savage TF, Zakrzewska EI, Andreasen JRJ (1997) The effects of feeding Bio-Mos®-supplemented diets to poults on performance and morphology of the small intestine. Poult Sci 73:139

    Google Scholar 

  45. Scupham AJ (2007) Succession in the intestinal microbiota of preadolescent turkeys. FEMS Microbiol Ecol 60:136–147

    Article  PubMed  CAS  Google Scholar 

  46. Sims MD, Dawson KA, Newman KE, Spring P, Hooge DM (2004) Effect of dietary mannan oligosaccharide, bacitracin methylene disalicylate, or both on the live performance and intestinal microbiology of turkeys. Poult Sci 83:1148–1154

    PubMed  CAS  Google Scholar 

  47. Sims, M. D., M. F. White, T. W. Alexander, T. Sefton, A. Connolly, and P. Spring. 1999. Evaluation of Bio-Mos fed alone and in combination with BMD to growing Tom turkeys. Poultry Science 78

  48. Singleton DR, Furlong MA, Rathbun SL, Whitman WB (2001) Quantitative comparisons of 16S rRNA gene sequence libraries from environmental samples. Appl Environ Microbiol 67:4374–4376

    Article  PubMed  CAS  Google Scholar 

  49. Stanczuk J, Zdunczyk Z, Juskiewicz J, Jankowski J (2005) Indices of response of young turkeys to diets containing mannan oligosaccharide or inulin. Vet Zootech 31:98–101

    Google Scholar 

  50. Swann MM (1969) Report of the Joint Committee on the Use of Antibiotics in Animal Husbandry and Veterinary Medicine. HMSO, London

    Google Scholar 

  51. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  52. Thakuria D, Schmidt O, Liliensiek A-K, Egan D, Doohan FM (2009) Field preservation and DNA extraction methods for intestinal microbial diversity analysis in earthworms. J Microbiol Methods 76:226–233

    Article  PubMed  CAS  Google Scholar 

  53. Thakuria D, Schmidt O, Mac Siúrtáin M, Egan D, Doohan FM (2008) Importance of DNA quality in comparative soil microbial community structure analyses. Soil Biol Biochem 40:1390–1403

    Article  CAS  Google Scholar 

  54. Thomke S, Elwinger K (1998) Growth promotants in feeding pigs and poultry. I. Growth and feed efficiency responses to antibiotic growth promotants. Ann Zootech 47:85–97

    Article  CAS  Google Scholar 

  55. Torsvik V, Goksoyr J, Daae FL (1990) High diversity in DNA of soil bacteria. Appl Environ Microbiol 56:782–787

    PubMed  CAS  Google Scholar 

  56. Wang M, Ahrne S, Jeppsson B, Molin G (2005) Comparison of bacterial diversity along the human intestinal tract by direct cloning and sequencing of 16S rRNA genes. FEMS Micro Ecol 54:219–231

    Article  CAS  Google Scholar 

  57. Yang Y, Iji PA, Choct M (2009) Dietary modulation of gut microflora in broiler chickens: a review of the role of six kinds of alternatives to in-feed antibiotics. World's Poult Sci J 65:97–114

    Article  Google Scholar 

  58. Zdunczyk Z, Juskiewicz J, Jankowski J, Biedrzycka E, Koncicki A (2005) Metabolic response of the gastrointestinal tract of turkeys to diets with different levels of mannan-oligosaccharide. Poult Sci 84:903–909

    PubMed  CAS  Google Scholar 

  59. Zhu XY, Zhong T, Pandya Y, Joerger RD (2002) 16S rRNA-based analysis of microbiota from the cecum of broiler chickens. Appl Environ Microbiol 68:124–137

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Alltech Biotechnology, Sarney, Summerhill Road, Dunboyne, Co. Meath, Ireland

    A. Corrigan, K. Horgan & R. A. Murphy

  2. School of Biology and Environmental Science, Ardmore House, University College Dublin, Belfield, Dublin, Ireland

    N. Clipson

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  1. A. Corrigan
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Correspondence to A. Corrigan.

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Corrigan, A., Horgan, K., Clipson, N. et al. Effect of Dietary Prebiotic (Mannan Oligosaccharide) Supplementation on the Caecal Bacterial Community Structure of Turkeys. Microb Ecol 64, 826–836 (2012). https://doi.org/10.1007/s00248-012-0046-6

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