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Modulatory Effects of Deltamethrin on Antioxidant Defense Mechanisms and Lipid Peroxidation in Carassius auratus gibelio Liver and Intestine

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Abstract

Pyrethroids, such as deltamethrin, are toxic substances that lead to generation of reactive oxygen species, which harm living organisms. We assessed the level and patterns of imbalance evolved by a single dose of 2 μg/L deltamethrin on the lipid peroxidation (LPO) and the antioxidant defense system of Carassius auratus gibelio liver and intestine, and monitored the recovery dynamics of these parameters during a 14-day post-exposure period. LPO and antioxidative defense mechanisms displayed different responses in the investigated tissues. Sudden increase of LPO in the liver, persisting at this elevated level throughout the test period, was observed on the third day post-exposure, while in the intestine significant enhancement of this parameter was recorded from the seventh day. Reduced glutathione (GSH) showed a transient increase in the liver, and was depleted in the intestine by the second day of exposure, with signs of recovery by the end of the experimental tenure. In the liver of fish a temporary inhibition of superoxide dismutase (SOD) and catalase (CAT) activity, and activation of glutathione peroxidase (GPX), glutathione transferase (GST), and glutathione reductase (GR) enzymes was observed, with maximum thresholds recorded on the third and second days, respectively. In the intestine a relevant increase in CAT and GST activity up to the second day and almost complete recovery by the end of the experiment was recorded, while for GR a continuous enhancement was apparent.

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References

  • Aebi H (1984) Catalase in vitro. In: Packer L (ed) Methods in enzymology, vol 105. Academic, New York, pp 673–684

    Google Scholar 

  • Ansari R, Kaur M, Ahmad F, Rahman S, Rashid H, Islam F, Raissudin S (2009) Genotoxic and oxidative stress-inducing effects of deltamethrin in the erythrocytes of a freshwater biomarker fish species, Channa punctata Bloch. Environ Toxicol 24:429–436

    Article  CAS  Google Scholar 

  • Atif F, Parvez S, Pandey S, Ali M, Kaur M, Rehman H, Khan HA, Raisuddin S (2005) Modulatory effect of cadmium exposure on deltemethrin-induced oxidative stress in Channa punctata Bloch. Arch Environ Contam Toxicol 49:371–377

    Article  CAS  Google Scholar 

  • Bainy ACD, Saito E, Carvalho PSM, Junqueira VBC (1996) Oxidative stress in gill, erythrocytes, liver and kidney of Nile tilapia (Oreochromis niloticus) from a polluted site. Aquat Toxicol 34:151–162

    Article  CAS  Google Scholar 

  • Bálint T, Ferenczy J, Kátai F, Kiss I, Kráczer L, Kufesák O, Láng G, Polyhos C, Szabó I, Szegletes T, NemcsóK J (1997) Similarities and differences between the massive eel (Anguilla Anguilla L.) devastations that occurred in Lake Balaton in 1991 and 1995. Ecotoxicol Environ Saf 37:17–23

    Article  Google Scholar 

  • Beutler E (1984) Glutathione peroxidase. In: Beutler E (ed) Red cell metabolism: a manual of biochemical methods. Grune & Stratton, New York, pp 74–76

    Google Scholar 

  • Bradberry SM, Cage SA, Proudfoot AT, Vale JA (2005) Poisoning due to pyrethroids. Toxicol Rev 24:93–106

    Article  CAS  Google Scholar 

  • Bradbury SP, Coats JR (1989) Comparative toxicology of the pyrethroid insecticides. Rev Environ Contam Toxicol 108:133–177

    CAS  Google Scholar 

  • Catala A (2006) An overview of lipid peroxidation with emphasis in outer segments of photoreceptors and the chemiluminescence assay. Int J Biochem Cell Biol 38:1482–1495

    Article  CAS  Google Scholar 

  • Dabrowski JM, Peall SKC, Reinecke AJ, Liess M, Schultz R (2002) Runoff-related pesticide input into Lourens Rive, South Africa: basic data for exposure assessment and risk mitigation at the catchment scale. Water Air Soil Pollut 135:265–283

    Article  CAS  Google Scholar 

  • Das R, Steege A, Baron S, Beckman J, Harrison R (2001) Pesticide-related illness among migrant farm workers in the United States. Int J Occup Environ Health 7:303–312

    CAS  Google Scholar 

  • Demoute JP (1989) A brief review of the environmental fate and metabolism of pyrethroids. Pest Sci 27:375–385

    Article  CAS  Google Scholar 

  • Draper HH, Hadley M (1990) Malondialdehyde determination as index of lipid peroxidation. In: Packer L, Glazer AN (eds) Methods in enzymology, vol 186. Academic, San Diego, pp 421–431

    Google Scholar 

  • Eells JT, Rasmussen JL, Bandettini PA, Propp JM (1993) Differences in the neuroexcitatory actions of pyrethroid insecticides and sodium specific neurotoxins in rat and trout brain synaptosomes. Toxicol Appl Pharmacol 123:107–119

    Article  CAS  Google Scholar 

  • Fatima M, Ahmed I, Sayeed I, Achar M, Raisuddin S (2000) Polluant induced overactivation of phagocytes is concomitantly associated with peroxidation of phagocytes in fish tissue. Aquat Toxicol 49:243–250

    Article  CAS  Google Scholar 

  • Goldberg DM, Spooner RJ (1983) Glutathione reductase. In: Bergmayer HU (ed) Methods of enzymatic analysis, 3rd edn. Verlag Chemie, Dearfield Beach, pp 258–265

    Google Scholar 

  • Habig WH, Pabst MJ, Jakoby W (1974) Glutathione-S-transferase. The first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139

    CAS  Google Scholar 

  • Hai DQ, Varga SI, Matkovics B (1997) Organophosphate effects on antioxidant system of carp (Cyprinus carpio) and catfish (Ictalurus neubulosus). Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 117:83–88

    Article  CAS  Google Scholar 

  • Hasspielar BM, Behar JV, DiGiulio RT (1994) Glutathione dependent defense in channel catfish (Ictalurus punctatus) and brown bullhead (Ameuirus nebulosus). Ecotoxicol Environ Saf 28:82–90

    Article  Google Scholar 

  • Haya K (1989) Toxicity of pyrethroid insecticides to fish. Environ Toxicol Chem 8:381–391

    Article  CAS  Google Scholar 

  • Jones DP, Eklow L, Thor H, Orrenius S (1981) Metabolism of hydrogene peroxide in isolated hepatocytes. Relative contribution of catalase and glutathione peroxidase in decomposition of endogenously generated hydrogen peroxide. Arch Biochem Biophys 210:505–516

    Article  CAS  Google Scholar 

  • Kehrer JP (1993) Free radicals as mediators in tissue injury and disease. Crit Rev Toxicol 23:21–48

    Article  CAS  Google Scholar 

  • Kono NS, Fridovich I (1982) Superoxide radical inhibits catalase. J Biol Chem 257:5751–5754

    CAS  Google Scholar 

  • Kontreczky C, Farkas A, Nemcsók J, Salánki J (1997) Short-and long-term effects of deltamethrin on filtering activity of freshwater mussel (Anodonta cygnea L.). Ecotoxicol Environ Saf 38:195–199

    Article  CAS  Google Scholar 

  • Kőprűcű SS, Kőprűcű K, Ural MS (2006) Acute toxicity of the synthetic pyrethroid deltamethrin to fingerling European catfish, Silurus glanis L. Bull Environ Contam Toxicol 76:59–65

    Article  CAS  Google Scholar 

  • Lenártová V, Holoská K, Pedrajas J-R, Martinez-Lara E, Peinado J, Lopez-Barea J, Rosived I, Košǘth P (1997) Antioxidant and detoxifying fish enzymes as biomarkers of river pollution. Biomarkers 2:247–252

    Article  Google Scholar 

  • Lima PL, Benassi JC, Pedrosa RC, Dal Magro J, Oliviera TB, Wilhem-Filho D (2005) Time course variations of DNA damage and biomarkers of oxidative stress in tilapia (Oreochromis niloticus) exposed to effluents of a swine industry. Arch Environ Contam Toxicol 49:1–10

    Article  CAS  Google Scholar 

  • Lowry OH, Rosenbrough NJ, Farr AL, Randall BJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Marks DB, Marks AD, Smith CM (1996) Oxygen metabolism and oxygen toxicity. In: Velker J (ed) Basic medical biochemistry: a clinical approach. Williams and Wilkins, Baltimore, pp 327–340

  • Martinez-Alvarez RM, Morales AE, Sanz A (2005) Antioxidant defenses in fish: biotic and abiotic factors. Rev Fish Biol Fisher 15:75–88

    Article  Google Scholar 

  • Mesters R, Mesters G (1992) Deltamethrin: uses and environmental safety. Rev Environ Contam Toxicol 124:1–18

    Google Scholar 

  • Milam CD, Faris JL, Wilhide JD (2000) Evaluating mosquito control pesticides for effect on target and non-target organism. Arch Environ Contam Toxicol 39:324–328

    Article  CAS  Google Scholar 

  • Paoletti F, Mocali A (1990) Determination of superoxide dismutase activity by purely chemical system based on NAD(P)H oxidation. In: Packer L, Glazer AN (eds) Methods in enzymology, vol 186. Academic, San Diego, pp 209–220

    Google Scholar 

  • Pickett CB, Lu AYH (1989) Glutathione S-transferase: gene structure, regulation and biological function. Annu Rev Biochem 58:743–764

    Article  CAS  Google Scholar 

  • Prohaska JR (1980) The glutathione peroxidase activity of glutathione S-transferases. Biochem Biophys Acta 611:87–98

    CAS  Google Scholar 

  • Rehman H, Ali M, Atif F, Kaur M, Bhatia K, Raisuddin S (2006) The modulatory effect of deltamethrin on antioxidants in mice. Clin Chem Acta 369:61–65

    Article  CAS  Google Scholar 

  • Sayeed I, Parvez S, Pandey S, Bin-Hafeez B, Haque R, Raisuddin S (2003) Oxidative stress biomarkers of exposure to deltamethrin in freshwater fish, Channa punctatus Bloch. Ecotoxicol Environ Saf 56:295–301

    Article  CAS  Google Scholar 

  • Scultz R (2004) Field studies on exposure, effects, and risk mitigation of aquatic nonpoint-source insecticide pollution. J Environ Qual 33:419–448

    Google Scholar 

  • Sedlak J, Lindsey RH (1968) Estimation of total, protein bound and nonprotein sulfhydryl groups in tissues with Ellman’s reagent. Anal Biochem 25:192–205

    Article  CAS  Google Scholar 

  • Sies H, Cadenas E (1985) Oxidative stress: damage to intact cells and organs. Philos Trans R Soc Lond B Biol Sci 311:617–631

    Article  CAS  Google Scholar 

  • Srivastav AK, Srivastava SK, Srivastav SK (1997) Impact of deltamethrin on serum calcium and inorganic phosphate of freshwater catfish, Heteropneustes fossilis. Bull Environ Contam Toxicol 59:841–846

    Article  CAS  Google Scholar 

  • Stadtman ER, Levine RL (2000) Protein oxidation. Ann NY Acad Sci 899:191–208

    CAS  Google Scholar 

  • Stephensen E, Sturve J, Forlin L (2002) Effects of redox cycling compounds on glutathione content and activity of glutathione-related enzymes in rainbow trout liver. Comp Biochem Physiol C Toxicol Pharmacol 133:435–442

    Google Scholar 

  • Turnbull AB, Harrison RM, Williams RJ, Matthiessen P, Brooke DN, Sheahan DA, Mills M (2007) Assessment of the fate of selected adsorptive pesticides at ADAS Rosemaund. Water Environ J 11:24–39

    Article  Google Scholar 

  • Tuzmen N, Candan N, Kaya E, Demiryas N (2008) Biochemical effects of chlorpyrifos and deltamethrin on altered antioxidative defense mechanisms and lipid peroxidation in rat liver. Cell Biochem Funct 26:119–124

    Article  CAS  Google Scholar 

  • Vais H, Williamson MS, Devonshire AL, Usherwood PN (2001) The molecular interactions of pyrethroid insecticides with insect and mammalian sodium channels. Pest Manag Sci 57:877–888

    Article  CAS  Google Scholar 

  • Van der Heuvel MO, Clarck DG, Fielder RI, Koundakjian PP, Oliver GI, Pelling D, Tomlison N, Walker AP (1990) The international validation of a fixed-dose procedure as an alternative to the classical LD50 test. Food Chem Toxicol 28:469–482

    Article  Google Scholar 

  • Velisek J, Dobsikova R, Svobodova Z, Modra H, Luskova V (2006) Effect of deltamethrin on the biochemical profile of common carp (Cyprinus carpio L.). Bull Environ Contam Toxicol 76:992–998

    Article  CAS  Google Scholar 

  • Viran R, Unlü Erkoc F, Polat H, Kocak O (2003) Investigation of acute toxicity of deltamethrin on guppies (Poecilia reticulate). Ecotoxicol Environ Saf 55:82–85

    Article  CAS  Google Scholar 

  • Yadav RS, Sampath RR, Sharma VP (2001) Deltamethrin treated bednets for control of malaria transmitted by Anopheles culicifacies (Diptera: Calicidae) in India. J Med Entomol 38:613–622

    Article  CAS  Google Scholar 

  • Yildirim MZ, Benli AC, Selvi M, Ozkul A, Erkoc F, Kocak O (2006) Acute toxicity, behavioral changes, and histopathological effects of deltamethrin on tissues (gills, liver, brain, spleen, kidney, muscle skin) of Nile tilapia (Oreochromis niloticus L.) fingerlings. Environ Toxicol 21:614–620

    Article  CAS  Google Scholar 

  • Yousef MI, Awad TI, Mohamed EH (2006) Deltamethrin-induced oxidative damage and biochemical alterations in rat and its attenuation by vitamin E. Toxicology 227:240–247

    Article  CAS  Google Scholar 

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Acknowledgments

This study was financially supported by the National Research Council of Higher Education, Romania, grant numbers PED 465/2003 and COST B35/2006. We also thank Prof. Radu Burlacu for his advice concerning statistical analysis.

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  1. Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Spl. Independentei, 050095, Bucharest, Romania

    Diana Dinu, Diana Marinescu, Maria Cristina Munteanu, Andreea Cristina Staicu, Marieta Costache & Anca Dinischiotu

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  1. Diana Dinu
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  2. Diana Marinescu
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Correspondence to Anca Dinischiotu.

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Dinu, D., Marinescu, D., Munteanu, M.C. et al. Modulatory Effects of Deltamethrin on Antioxidant Defense Mechanisms and Lipid Peroxidation in Carassius auratus gibelio Liver and Intestine. Arch Environ Contam Toxicol 58, 757–764 (2010). https://doi.org/10.1007/s00244-009-9401-0

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