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Factors Affecting Antioxidant Response in Fish from a Long-term Mercury-Contaminated Reservoir

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Abstract

The objective of this work was to evaluate antioxidant defence and oxidative damage in organs (liver, gills, kidney, and brain) of five fish species (Aspius aspius, Esox lucius, Sander lucioperca, Abramis brama, Rutilus rutilus) from the long-term mercury-contaminated Skalka Reservoir in the Czech Republic. Special emphasis was placed on a comprehensive assessment of the factors that may affect the antioxidant response to mercury in fish. Antioxidant enzymes (glutathione reductase, glutathione peroxidase, and glutathione-S-transferase) did not significantly respond to mercury contamination. Levels of the analysed enzymes and oxidative damage to lipids were predominantly determined by a separate organ factor or species factor, or by the combination of both (p < 0.001). Levels of total glutathione and the reduced/oxidized glutathione ratio were influenced by mercury contamination in combination with their specific organ distribution (p < 0.001). Our results suggest that species and type of organ alone or in combination are more important factors than chronic exposure to mercury contamination with respect to effects on antioxidant defence in fish under field conditions. Our findings suggest that the main antioxidant defensive mechanism in fish from the studied long-term mercury contaminated site was the inter-tissue distribution of glutathione.

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Acknowledgments

This work was financially supported by IGA VFU 88/2011/FVHE. The authors thank Matthew Nicholls for proofreading this paper.

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Authors and Affiliations

  1. Department of Veterinary Public Health and Animal Welfare, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho tr. 1/3, 612 42, Brno, Czech Republic

    M. Sevcikova, H. Modra, J. Blahova, R. Dobsikova & Z. Svobodova

  2. Institute of Biostatistics and Analyses, Masaryk University, Kamenice 3, 625 000, Brno, Czech Republic

    J. Kalina

  3. Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic

    O. Zitka & R. Kizek

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  1. M. Sevcikova
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  2. H. Modra
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  3. J. Blahova
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  4. R. Dobsikova
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  5. J. Kalina
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  6. O. Zitka
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  7. R. Kizek
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  8. Z. Svobodova
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Correspondence to M. Sevcikova.

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Sevcikova, M., Modra, H., Blahova, J. et al. Factors Affecting Antioxidant Response in Fish from a Long-term Mercury-Contaminated Reservoir. Arch Environ Contam Toxicol 69, 431–439 (2015). https://doi.org/10.1007/s00244-015-0213-0

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