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Validation and Application of a Method for the Determination of Total Chromium in Rat Tissues by Inductively Coupled Plasma Mass Spectrometry

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Abstract

The validation of a method for the determination of chromium (Cr) in F-344/N rat tissues by inductively coupled plasma-mass spectrometry is described. Samples were analyzed after a rapid, open-vessel microwave digestion procedure. Performance of the method was evaluated using kidney tissue across a concentration range of 0.50–5.00 μg Cr/g tissue. Data for method linearity, accuracy, precision, digest stability, and storage stability are presented along with limits of detection and quantitation data. Data from a method cross-validation for B6C3F1 mouse kidney tissue are also presented. After validation, the method was applied to analyze samples collected in support of two chronic toxicity and carcinogenesis studies conducted by the National Toxicology Program.

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Acknowledgements

This work was funded in full by the National Institute of Environmental Health Sciences, National Institute of Health, Contract Nos. N01-ES-05455 and N01-ES-65554.

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Authors and Affiliations

  1. RTI International, Research Triangle Park, NC, 27709, USA

    Keith E. Levine, Glenn T. Ross, Amal S. Essader, Frank X. Weber, Peter M. Grohse, Reshan A. Fernando & Lisa S. Milstein

  2. National Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, NC, 27709, USA

    Matthew D. Stout, Michelle J. Hooth & Bradley J. Collins

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  1. Keith E. Levine
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  2. Matthew D. Stout
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  3. Glenn T. Ross
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  4. Amal S. Essader
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  5. Frank X. Weber
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  6. Peter M. Grohse
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  7. Reshan A. Fernando
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  8. Lisa S. Milstein
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  9. Michelle J. Hooth
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  10. Bradley J. Collins
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Correspondence to Keith E. Levine.

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Levine, K.E., Stout, M.D., Ross, G.T. et al. Validation and Application of a Method for the Determination of Total Chromium in Rat Tissues by Inductively Coupled Plasma Mass Spectrometry. Arch Environ Contam Toxicol 58, 883–891 (2010). https://doi.org/10.1007/s00244-009-9397-5

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  • DOI: https://doi.org/10.1007/s00244-009-9397-5

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