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Intraluminal measurement of papillary duct urine pH, in vivo: a pilot study in the swine kidney

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An Erratum to this article was published on 05 March 2016

Abstract

We describe the in vivo use of an optic-chemo microsensor to measure intraluminal papillary duct urine pH in a large mammal. Fiber-optic pH microsensors have a tip diameter of 140-µm that allows insertion into papillary Bellini ducts to measure tubule urine proton concentration. Anesthetized adult pigs underwent percutaneous nephrolithotomy to access the lower pole of the urinary collecting system. A flexible nephroscope was advanced towards an upper pole papilla with the fiber-optic microsensor contained within the working channel. The microsensor was then carefully inserted into Bellini ducts to measure tubule urine pH in real time. We successfully recorded tubule urine pH values in five papillary ducts from three pigs (1 farm pig and 2 metabolic syndrome Ossabaw pigs). Our results demonstrate that optical microsensor technology can be used to measure intraluminal urine pH in real time in a living large mammal. This opens the possibility for application of this optical pH sensing technology in nephrolithiasis.

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Acknowledgments

Drs. Ehud Gnessin and Jessica Mandeville aided in the percutaneous access surgery with JEL inserting fiber-optic microsensors into Bellini ducts. RKH operated and evaluated the performance of the fiber-optic phase detection system. The authors are grateful to Dr. James A. McAteer for insightful comments on the manuscript, and to Philip M. Blomgren for preparing illustrations.

Funding

Supported by Public Health Service grants P01-DK56788 and P01-DK43881.

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology (RKH, SBB, APE, BAC, CDJ), Indiana University School of Medicine, Indianapolis, IN, USA

    Rajash K. Handa, James E. Lingeman, Sharon B. Bledsoe, Andrew P. Evan, Bret A. Connors & Cynthia D. Johnson

  2. Department of Urology (JEL), Indiana University Health at Methodist Hospital, Indianapolis, IN, USA

    Rajash K. Handa

Authors
  1. Rajash K. Handa
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  2. James E. Lingeman
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  3. Sharon B. Bledsoe
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  4. Andrew P. Evan
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  5. Bret A. Connors
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  6. Cynthia D. Johnson
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Corresponding author

Correspondence to Rajash K. Handa.

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Conflict of interest

Authors RKH, SBB, APE, BAC and CDJ declare no conflict of interest; JEL has investment interests with Midwest Mobile Lithotripsy and Midstate Mobile Lithotripsy.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Handa, R.K., Lingeman, J.E., Bledsoe, S.B. et al. Intraluminal measurement of papillary duct urine pH, in vivo: a pilot study in the swine kidney. Urolithiasis 44, 211–217 (2016). https://doi.org/10.1007/s00240-015-0834-9

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  • DOI: https://doi.org/10.1007/s00240-015-0834-9

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