Skip to main content

Advertisement

Log in

Immediate cell-supplemented lipotransfer (iCSL)

  • Original Paper
  • Published:
European Journal of Plastic Surgery Aims and scope Submit manuscript

Abstract

Studies have shown that adipose-derived stem cells may improve neovascularization, graft take, and much more. Many strategies have been developed in the last years to isolate and take advantage of these cells for different applications in many specialties. Immediate cell-supplemented lipotransfer (iCSL) is a reproducible and immediate approach that consists in isolating a great number of stem cells from the harvested fat and injecting the suspension with processed adipose tissue as a scaffold in the same procedure, in a short time and cost-effective manner. In this article, the authors present iCSL throughout its processing method, its advantages and current applications in plastic and reconstructive surgery, and its potential applications in all fields of regenerative medicine

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Zuk PA, Zhu M, Ashjian P et al (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13:4279–4295

    Article  PubMed  CAS  Google Scholar 

  2. Lovell-Badge R (2001) The future for stem cell research. Nature 414:88–91

    Article  PubMed  CAS  Google Scholar 

  3. Condé-Green A, Pitanguy I et al (2010) Influence of decantation, washing and centrifugation on adipocyte and mesenchymal stem cell content of aspirated adipose tissue: a comparative study. J Plast Reconstr Aesthet Surg 63(8):1375–1381

    Article  PubMed  Google Scholar 

  4. Yoshimura K, Sato K, Aoi N et al (2008) Cell-assisted lipotransfer for facial atrophy: efficacy of clinical use of adipose-derived stem cells. Dermatol Surg 34(9):1178–1185

    Article  PubMed  CAS  Google Scholar 

  5. Tat PA, Sumer H, Jones KL et al (2010) The efficient generation of induced pluripotent stem (iPS) cells from adult mouse adipose tissue-derived and neural stem cells. Cell Transplant 19(5):525–536

    Article  PubMed  Google Scholar 

  6. Yoshimura K, Shigeura T, Matsumoto D et al (2006) Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol 208:64–76

    Article  PubMed  CAS  Google Scholar 

  7. Coleman SR (2006) Facial augmentation with structural fat grafting. Clin Plast Surg 33:567–577

    Article  PubMed  Google Scholar 

  8. Illouz YG (1986) The fat cell graft: a new technique to fill depressions. Plast Reconstr Surg 78:122e3

    Article  Google Scholar 

  9. Cao Y (2007) Angiogenesis modulates adipogenesis and obesity. J Clin Invest 117(9):2362–2368

    Article  PubMed  CAS  Google Scholar 

  10. Blanton MW, Hadad L, Johnstone BH (2009) Adipose stromal cells and platelet-rich plasmatherapies synergistically increase revascularization during wound healing. Plast Reconstr Surg 123(2S):56S–64S

    Article  PubMed  CAS  Google Scholar 

  11. Gonda K, Shigeura T, Sato T et al (2008) Preserved proliferative capacity and multipotency of human adipose-derived stem cells after long-term cryopreservation. Plast Reconstr Surg 121(2):401–410

    Article  PubMed  CAS  Google Scholar 

  12. Gu H, Guo F, Zhou X et al (2011) The stimulation of osteogenic differentiation of human adipose-derived stem cells by ionic products from akermanite dissolution via activation of the ERK pathway. Biomaterials 32(29):7023–7033

    Article  PubMed  CAS  Google Scholar 

  13. Scholz T, Sumarto A, Krichevsky A (2011) Neuronal differentiation of human adipose tissue-derived stem cells for peripheral nerve regeneration in vivo. Arch Surg 146(6):666–674

    Article  PubMed  Google Scholar 

  14. Levi B, Nelson ER, Li S et al (2011) Dura mater stimulates human adipose-derived stromal cells to undergo bone formation in mouse calvarial defects. Stem Cells 29(8):1241–1255

    Article  PubMed  CAS  Google Scholar 

  15. Condé-Green A, Borojevic R, Pitanguy I et al (2010) Effects of centrifugation on cell composition and viability of aspirated adipose tissue processed for transplantation. Aesthet Surg J 30(2):249–255

    Article  PubMed  Google Scholar 

  16. Lu F, Mizuno H, Uysal C et al (2008) Improved viability of random pattern skin flaps through the use of adipose-derived stem cells. Plast Reconstr Surg 121(1):50–58

    Article  PubMed  CAS  Google Scholar 

  17. Uysal AC, Mizuno H, Tobita M et al (2009) The effect of adipose-derived stem cells on ischemiareperfusion injury: immunohistochemical and ultrastructural evaluation. Plast Reconstr Surg 124(3):804–815

    Article  PubMed  CAS  Google Scholar 

  18. Mizuno H, Itoi Y, Kawahara S et al (2008) In vivo adipose tissue regeneration by adipose-derived stromal cells isolated from GFP transgenic mice. Cells Tissues Organs 187:177–185

    Article  PubMed  Google Scholar 

  19. Levi B, James AW, Nelson ER et al (2011) Studies in adipose-derived stromal cells: migration and participation in repair of cranial injury after systemic injection. Plast Reconstr Surg 127(3):1130–1140

    Article  PubMed  CAS  Google Scholar 

  20. Sajjadian A, Tandav MK (2007) Treating facial soft tissue deficiency: fat grafting and adipose derived stem cell tissue engineering. Aesthet Surg J 27(1):100–104

    Article  PubMed  CAS  Google Scholar 

  21. Tobita M, Orbay H, Mizuno H (2011) Adipose-derived stem cells: current findings and future perspectives. Discov Med 11(57):160–170

    PubMed  Google Scholar 

  22. Stoisch MS, Mao JJ (2007) Adipose tissue engineering from human adult stem cells: clinical implications in plastic and reconstructive surgery. Plast Reconstr Surg 119(1):71–83

    Article  Google Scholar 

  23. Condé Green A (2010) Optimized autologous fat grafting: effects of harvesting, manipulation on live mesenchymal stromal cells. Plast Reconstr Surg 126:57–58, Abstract supplement. Presented at the American Society of Plastic Surgeons meeting, Plastic Surgery 2010 in Toronto, Canada

    Article  Google Scholar 

  24. Aiba-Kojima E, Tsuno NH, Inoue K et al (2007) Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture. Wound Repair Regen 15(4):511–520

    Article  PubMed  Google Scholar 

  25. Altman AM, Abdul Khalek FJ, Seidensticker M et al (2010) Human tissue-resident stem cells combined with hyaluronic acid gel provide fibrovascular-integrated soft tissue augmentation in a murine photoaged skin model. Plast Reconstr Surg 125(1):63–73

    Article  PubMed  CAS  Google Scholar 

  26. Ashjian PH, De Ugarte D, Katz AJ et al (2003) Lipoplasty: from body contouring to tissue engineering. Ann Plast Surg 50(2):215–219

    Article  PubMed  Google Scholar 

  27. Piasecki JH, Moreno K, Gutowski KA (2008) Beyond the cells: scaffold matrix character affects the in vivo performance of purified adipocyte fat grafts. Aesthet Surg J 28(3):306–312

    Article  PubMed  Google Scholar 

  28. Lu F, Li J, Gao J et al (2009) Improvement of the survival of human autologous fat transplantation by using VEGF-transfected adipose-derived stem cells. Plast Reconstr Surg 124(5):1437–1446

    Article  PubMed  CAS  Google Scholar 

  29. Rubin JP, Agha-Mohammadi S (2003) Mesenchymal stem cells: aesthetic applications. Aesthet Surg J 23(6):504–506

    Article  PubMed  Google Scholar 

  30. Masuda T, Furue M, Matsuda T (2004) Photocured, styrenated, gelatin-based microspheres for de novo adipogenesis through corelease of basic fibroblast growth factor, insulin and insulin-like growth factor. Tissue Eng 10:523–535

    Article  PubMed  CAS  Google Scholar 

  31. Kitamura K, Kajitani K, Hedrick M, Sugimachi K (2007) Stem cell augmented reconstruction: a new hope for reconstruction after breast conservation therapy. Breast Cancer Res Treat 106(suppl 1), Abstract 4071

    Google Scholar 

  32. Yoshimura K, Sato K, Aoi N et al (2008) Cell-assisted lipotransfer for cosmetic breast augmentation: supportive use of adipose-derived stem/stromal cells. Aesthetic Plast Surg 32:48–55, Discussion, 56–57

    Article  PubMed  Google Scholar 

  33. Behr B, Ko SH, Wong VW et al (2010) Stem cells. Plast Reconstr Surg 126:1163–1171

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to express their appreciation to Dr. John McLenithan, Laurent Xavier, and Pedro Figueiredo for their help in the preparation of this work.

Disclosures

The authors declare no conflicts of interests with respect to the authorship and/or publication of this article.

Funding

The authors received no financial support for the research and/or authorship of this article.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Alexandra Condé-Green.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Condé-Green, A., Lamblet, H. Immediate cell-supplemented lipotransfer (iCSL). Eur J Plast Surg 35, 373–378 (2012). https://doi.org/10.1007/s00238-011-0651-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00238-011-0651-5

Keywords

Navigation