Abstract
Studies have shown that adipose-derived stem cells may improve neovascularization, graft take, and much more. Many strategies have been developed in the last years to isolate and take advantage of these cells for different applications in many specialties. Immediate cell-supplemented lipotransfer (iCSL) is a reproducible and immediate approach that consists in isolating a great number of stem cells from the harvested fat and injecting the suspension with processed adipose tissue as a scaffold in the same procedure, in a short time and cost-effective manner. In this article, the authors present iCSL throughout its processing method, its advantages and current applications in plastic and reconstructive surgery, and its potential applications in all fields of regenerative medicine
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References
Zuk PA, Zhu M, Ashjian P et al (2002) Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 13:4279–4295
Lovell-Badge R (2001) The future for stem cell research. Nature 414:88–91
Condé-Green A, Pitanguy I et al (2010) Influence of decantation, washing and centrifugation on adipocyte and mesenchymal stem cell content of aspirated adipose tissue: a comparative study. J Plast Reconstr Aesthet Surg 63(8):1375–1381
Yoshimura K, Sato K, Aoi N et al (2008) Cell-assisted lipotransfer for facial atrophy: efficacy of clinical use of adipose-derived stem cells. Dermatol Surg 34(9):1178–1185
Tat PA, Sumer H, Jones KL et al (2010) The efficient generation of induced pluripotent stem (iPS) cells from adult mouse adipose tissue-derived and neural stem cells. Cell Transplant 19(5):525–536
Yoshimura K, Shigeura T, Matsumoto D et al (2006) Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol 208:64–76
Coleman SR (2006) Facial augmentation with structural fat grafting. Clin Plast Surg 33:567–577
Illouz YG (1986) The fat cell graft: a new technique to fill depressions. Plast Reconstr Surg 78:122e3
Cao Y (2007) Angiogenesis modulates adipogenesis and obesity. J Clin Invest 117(9):2362–2368
Blanton MW, Hadad L, Johnstone BH (2009) Adipose stromal cells and platelet-rich plasmatherapies synergistically increase revascularization during wound healing. Plast Reconstr Surg 123(2S):56S–64S
Gonda K, Shigeura T, Sato T et al (2008) Preserved proliferative capacity and multipotency of human adipose-derived stem cells after long-term cryopreservation. Plast Reconstr Surg 121(2):401–410
Gu H, Guo F, Zhou X et al (2011) The stimulation of osteogenic differentiation of human adipose-derived stem cells by ionic products from akermanite dissolution via activation of the ERK pathway. Biomaterials 32(29):7023–7033
Scholz T, Sumarto A, Krichevsky A (2011) Neuronal differentiation of human adipose tissue-derived stem cells for peripheral nerve regeneration in vivo. Arch Surg 146(6):666–674
Levi B, Nelson ER, Li S et al (2011) Dura mater stimulates human adipose-derived stromal cells to undergo bone formation in mouse calvarial defects. Stem Cells 29(8):1241–1255
Condé-Green A, Borojevic R, Pitanguy I et al (2010) Effects of centrifugation on cell composition and viability of aspirated adipose tissue processed for transplantation. Aesthet Surg J 30(2):249–255
Lu F, Mizuno H, Uysal C et al (2008) Improved viability of random pattern skin flaps through the use of adipose-derived stem cells. Plast Reconstr Surg 121(1):50–58
Uysal AC, Mizuno H, Tobita M et al (2009) The effect of adipose-derived stem cells on ischemiareperfusion injury: immunohistochemical and ultrastructural evaluation. Plast Reconstr Surg 124(3):804–815
Mizuno H, Itoi Y, Kawahara S et al (2008) In vivo adipose tissue regeneration by adipose-derived stromal cells isolated from GFP transgenic mice. Cells Tissues Organs 187:177–185
Levi B, James AW, Nelson ER et al (2011) Studies in adipose-derived stromal cells: migration and participation in repair of cranial injury after systemic injection. Plast Reconstr Surg 127(3):1130–1140
Sajjadian A, Tandav MK (2007) Treating facial soft tissue deficiency: fat grafting and adipose derived stem cell tissue engineering. Aesthet Surg J 27(1):100–104
Tobita M, Orbay H, Mizuno H (2011) Adipose-derived stem cells: current findings and future perspectives. Discov Med 11(57):160–170
Stoisch MS, Mao JJ (2007) Adipose tissue engineering from human adult stem cells: clinical implications in plastic and reconstructive surgery. Plast Reconstr Surg 119(1):71–83
Condé Green A (2010) Optimized autologous fat grafting: effects of harvesting, manipulation on live mesenchymal stromal cells. Plast Reconstr Surg 126:57–58, Abstract supplement. Presented at the American Society of Plastic Surgeons meeting, Plastic Surgery 2010 in Toronto, Canada
Aiba-Kojima E, Tsuno NH, Inoue K et al (2007) Characterization of wound drainage fluids as a source of soluble factors associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture. Wound Repair Regen 15(4):511–520
Altman AM, Abdul Khalek FJ, Seidensticker M et al (2010) Human tissue-resident stem cells combined with hyaluronic acid gel provide fibrovascular-integrated soft tissue augmentation in a murine photoaged skin model. Plast Reconstr Surg 125(1):63–73
Ashjian PH, De Ugarte D, Katz AJ et al (2003) Lipoplasty: from body contouring to tissue engineering. Ann Plast Surg 50(2):215–219
Piasecki JH, Moreno K, Gutowski KA (2008) Beyond the cells: scaffold matrix character affects the in vivo performance of purified adipocyte fat grafts. Aesthet Surg J 28(3):306–312
Lu F, Li J, Gao J et al (2009) Improvement of the survival of human autologous fat transplantation by using VEGF-transfected adipose-derived stem cells. Plast Reconstr Surg 124(5):1437–1446
Rubin JP, Agha-Mohammadi S (2003) Mesenchymal stem cells: aesthetic applications. Aesthet Surg J 23(6):504–506
Masuda T, Furue M, Matsuda T (2004) Photocured, styrenated, gelatin-based microspheres for de novo adipogenesis through corelease of basic fibroblast growth factor, insulin and insulin-like growth factor. Tissue Eng 10:523–535
Kitamura K, Kajitani K, Hedrick M, Sugimachi K (2007) Stem cell augmented reconstruction: a new hope for reconstruction after breast conservation therapy. Breast Cancer Res Treat 106(suppl 1), Abstract 4071
Yoshimura K, Sato K, Aoi N et al (2008) Cell-assisted lipotransfer for cosmetic breast augmentation: supportive use of adipose-derived stem/stromal cells. Aesthetic Plast Surg 32:48–55, Discussion, 56–57
Behr B, Ko SH, Wong VW et al (2010) Stem cells. Plast Reconstr Surg 126:1163–1171
Acknowledgments
The authors would like to express their appreciation to Dr. John McLenithan, Laurent Xavier, and Pedro Figueiredo for their help in the preparation of this work.
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The authors declare no conflicts of interests with respect to the authorship and/or publication of this article.
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Condé-Green, A., Lamblet, H. Immediate cell-supplemented lipotransfer (iCSL). Eur J Plast Surg 35, 373–378 (2012). https://doi.org/10.1007/s00238-011-0651-5
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DOI: https://doi.org/10.1007/s00238-011-0651-5