Abstract
Amphipathic polymers known as “amphipols” provide a highly stabilizing environment for handling membrane proteins in aqueous solutions. A8-35, an amphipol with a polyacrylate backbone and hydrophobic grafts, has been extensively characterized and widely employed for structural and functional studies of membrane proteins using biochemical and biophysical approaches. Given the sensitivity of membrane proteins to their environment, it is important to examine what effects amphipols may have on the structure and dynamics of the proteins they complex. Here we present the first molecular dynamics study of an amphipol-stabilized membrane protein, using Escherichia coli OmpX as a model. We begin by describing the structure of the complexes formed by supplementing OmpX with increasing amounts of A8-35, in order to determine how the amphipol interacts with the transmembrane and extramembrane surfaces of the protein. We then compare the dynamics of the protein in either A8-35, a detergent, or a lipid bilayer. We find that protein dynamics on all accessible length scales is restrained by A8-35, which provides a basis to understanding some of the stabilizing and functional effects of amphipols that have been experimentally observed.
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Abbreviations
- 2D:
-
Two-dimensional
- A8-35:
-
A poly(sodium acrylate)-based amphipol comprising ~35 % of free carboxylates, ~25 % of octyl chains, ~40 % of isopropyl groups
- APol:
-
Amphipol
- CG:
-
Coarse-grain
- DDM:
-
n-Dodecyl-β-d-maltopyranoside
- DHPC:
-
Dihexanoylphosphatidylcholine
- DOPC:
-
Dioleoylphosphatidylcholine
- EM:
-
Electron microscopy
- FWHM:
-
Full width at half-maximum
- MD:
-
Molecular dynamics
- MP:
-
Membrane protein
- OmpX:
-
Outer membrane protein X from Escherichia coli
- PCA:
-
Principal component analysis
- RCG:
-
Reverse coarse-grain
- RMSF:
-
Root mean squared fluctuations
- SERCA1a:
-
The fast twitch sarcoplasmic calcium pump
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Acknowledgments
Particular thanks are due to L. J. Catoire, M. Tehei, and G. Zaccai for discussions about the manuscript, as well as to a referee for useful comments. Computational resources were provided by the Minnesota Supercomputing Institute (MSI). This work was also supported by the French Centre National de la Recherche Scientifique.
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Perlmutter, J.D., Popot, JL. & Sachs, J.N. Molecular Dynamics Simulations of a Membrane Protein/Amphipol Complex. J Membrane Biol 247, 883–895 (2014). https://doi.org/10.1007/s00232-014-9690-8
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DOI: https://doi.org/10.1007/s00232-014-9690-8