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Hollow Microneedle Arrays for Intradermal Drug Delivery and DNA Electroporation

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Abstract

The association of microneedles with electric pulses causing electroporation could result in an efficient and less painful delivery of drugs and DNA into the skin. Hollow conductive microneedles were used for (1) needle-free intradermal injection and (2) electric pulse application in order to achieve electric field in the superficial layers of the skin sufficient for electroporation. Microneedle array was used in combination with a vibratory inserter to disrupt the stratum corneum, thus piercing the skin. Effective injection of proteins into the skin was achieved, resulting in an immune response directed to the model antigen ovalbumin. However, when used both as microneedles to inject and as electrodes to apply the electric pulses, the setup showed several limitations for DNA electrotransfer. This could be due to the distribution of the electric field in the skin as shown by numerical calculations and/or the low dose of DNA injected. Further investigation of these parameters is needed in order to optimize minimally invasive DNA electrotransfer in the skin.

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Acknowledgments

Are addressed to the UE sixth framework program and the Angioskin consortium (LSH-2003-512127) that made this work possible.

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Authors and Affiliations

  1. Louvain Drug Research Institute, Unité de pharmacie galénique, Université Catholique de Louvain, Avenue E. Mounier 73/20, 1200, Brussels, Belgium

    Liévin Daugimont, Gaëlle Vandermeulen & Véronique Préat

  2. Département MN2S, Institut FEMTO-ST, UMR CNRS 6174, ENSMM, Besançon, France

    Nolwenn Baron & Gonzalo Cabodevila

  3. Faculty of Electrical Engineering, University of Ljubljana, Ljubljana, Slovenia

    Natasa Pavselj & Damijan Miklavcic

  4. CNRS UMR 8029, SATIE, ENS Cachan Bretagne, Bruz, France

    Marie-Caroline Jullien

  5. CNRS UMR 8203, Institut Gustave-Roussy, Villejuif and Université Paris-Sud, Orsay, France

    Lluis M. Mir

Authors
  1. Liévin Daugimont
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  2. Nolwenn Baron
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  3. Gaëlle Vandermeulen
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  4. Natasa Pavselj
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  5. Damijan Miklavcic
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  6. Marie-Caroline Jullien
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  7. Gonzalo Cabodevila
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  8. Lluis M. Mir
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  9. Véronique Préat
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Corresponding author

Correspondence to Véronique Préat.

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The first two authors contributed equally to this work.

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Daugimont, L., Baron, N., Vandermeulen, G. et al. Hollow Microneedle Arrays for Intradermal Drug Delivery and DNA Electroporation. J Membrane Biol 236, 117–125 (2010). https://doi.org/10.1007/s00232-010-9283-0

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  • DOI: https://doi.org/10.1007/s00232-010-9283-0

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