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Ontogeny of d-Mannose Transport and Metabolism in Rat Small Intestine

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Abstract

Oral mannose therapy is used to treat congenital disorders of glycosylation caused by a deficiency in phosphomannose isomerase. The segmental distribution and ontogenic regulation of d-mannose transport, phosphomannose isomerase, and phosphomannose mutase is investigated in the small intestine of fetuses, newborn, suckling, 1-month-old, and adult rats. The small intestine transports d-mannose by both Na+-dependent and Na+-independent transport mechanisms. The activities of both systems normalized to intestinal weight peak at birth and thereafter they decreased. In all the ages tested, the activity of the Na+-independent mechanism was higher than that of the Na+/mannose transport system. At birth, the Na+-independent d-mannose transport in the ileum was significantly higher than that in jejunum. Phosphomannose isomerase activity and mRNA levels increased at 1 month, and the values in the ileum were lower than in jejunum. Phosphomannose mutase activity in jejunum increased during the early stages of life, and it decreased at 1 month old, as does the amount of mannose incorporated into glycoproteins, whereas in the ileum, they were not affected by age. The phosphomannose isomerase/phosphomannose mutase activity ratio decreased at birth and during the suckling period, and increased at 1 month old. In conclusion, intestinal d-mannose transport activity and metabolism were affected by ontogeny and intestinal segment.

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Acknowledgments

This work was supported by grant MCyT-BFU 2006-00720. The group is member of the Network for Cooperative Research on Membrane Transport Proteins (REIT), cofunded by MCyT, Spain, and the European Regional Development Fund (ERDF) (grant BFU2007-30688-E/BFI).

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  1. Departamento de Fisiología y Zoología, Facultad de Farmacia, Universidad de Sevilla, C/Profesor García González, nº 2, 41012, Sevilla, Spain

    Mecedes Cano & Anunciación A. Ilundain

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  1. Mecedes Cano
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  2. Anunciación A. Ilundain
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Correspondence to Anunciación A. Ilundain.

Additional information

A preliminary report of some of these results was published in abstract form (Cano et al. 2005).

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Cano, M., Ilundain, A.A. Ontogeny of d-Mannose Transport and Metabolism in Rat Small Intestine. J Membrane Biol 235, 101–108 (2010). https://doi.org/10.1007/s00232-010-9259-0

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