Journal of Membrane Biology

, Volume 234, Issue 3, pp 217–225 | Cite as

Actin Microfilament Involved in Regulation of Pacemaking Activity in Cultured Interstitial Cells of Cajal from Murine Intestine

  • Zuo Yu Wang
  • Yan Fei Han
  • Xu Huang
  • Hong Li Lu
  • Xin Guo
  • Yong Chul Kim
  • Wen Xie Xu
Article

Abstract

The present study investigated the effect of actin microfilament structure on pacemaker currents and calcium oscillation in cultured murine intestinal interstitial cells of Cajal (ICCs) by whole-cell patch-clamp technique and calcium imaging technique. Cytochalasin B, a disruptor of actin microfilaments, decreased the amplitude and frequency of pacemaker currents from 491.32 ± 160.33 pA and 11.73 ± 0.79 cycles/min to 233.12 ± 92.00 pA and 10.29 ± 0.76 cycles/min. Cytochalasin B also decreased the amplitude and frequency of calcium oscillation from 0.32 ± 0.08 (ΔF/F0) and 2.75 ± 0.17 cycles/min to 0.02 ± 0.01 (ΔF/F0) and 1.20 ± 0.08 cycles/min. Phalloidin, a stabilizer of actin microfilaments, increased the amplitude and frequency of pacemaker currents from 751.79 ± 282.82 pA and 13.93 ± 1.00 cycles/min to 1234.34 ± 607.83 pA and 14.68 ± 1.00 cycles/min. Phalloidin also increased the amplitude and frequency of calcium oscillation from 0.26 ± 0.01 (ΔF/F0) and 2.27 ± 0.18 cycles/min to 0.43 ± 0.03 (ΔF/F0) and 2.87 ± 0.07 cycles/min. 2-Aminoethoxydiphenyl borane (2-APB), an IP3 receptor blocker, suppressed both pacemaker currents and calcium oscillations. 2-APB also blocked the phalloidin-induced increase in pacemaker currents and calcium oscillation. Ryanodine, an inhibitor of calcium-induced calcium release, did not affect pacemaker current but suppressed calcium oscillations. Ryanodine had no effect on altering phalloidin-induced increases in pacemaker current and calcium oscillation. These results suggest that actin microfilaments regulate pacemaker activity via the IP3-induced calcium release signaling pathway.

Keywords

Patch clamp technique Calcium imaging technique Interstitial cells of Cajal Actin microfilament Pacemaker current Calcium oscillation 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Zuo Yu Wang
    • 1
  • Yan Fei Han
    • 1
  • Xu Huang
    • 1
  • Hong Li Lu
    • 1
  • Xin Guo
    • 1
  • Yong Chul Kim
    • 2
  • Wen Xie Xu
    • 1
  1. 1.Department of PhysiologyShanghai Jiaotong University School of MedicineShanghaiChina
  2. 2.Department of PhysiologyChungbuk National University College of MedicineChungbukKorea

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