Abstract
Organic anion-transporting polypeptides (human, OATPs; other animals, Oatps; gene symbol, SLCO/Slco) form a transport protein superfamily that mediates the translocation of amphipathic substrates across the plasma membrane of animal cells. So far, OATPs/Oatps have been identified in human, rat and mouse tissues. In this study, we used bioinformatic tools to detect new members of the OATP/SLCO superfamily in nonmammalian species and to build models for the three-dimensional structure of OATPs/Oatps. New OATP/SLCO superfamily members, some of which form distinct novel families, were identified in chicken, zebrafish, frog, fruit fly and worm species. The lack of OATP/SLCO superfamily members in plants, yeast and bacteria suggests the emergence of an ancient Oatp protein in an early ancestor of the animal kingdom. Structural models were generated for the representative members OATP1B3 and OATP2B1 based on the known structures of the major facilitator superfamily of transport proteins. A model was also built for the large extracellular region between transmembrane helices 9 and 10, following the identification of a novel homology with the Kazal-type serine protease inhibitors. Along with the electrostatic potential and the conservation of key amino acid residues, we propose a common transport mechanism for all OATPs/Oatps, whereby substrates are translocated through a central, positively charged pore in a rocker-switch type of mechanism. Several amino acid residues were identified that may play crucial roles in the proposed transport mechanism.
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Acknowledgments
We thank Dr. Bruno Hagenbuch (Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center) and Dr. Bruno Stieger (Department of Clinical Pharmacology and Toxicology, University Hospital Zurich) for their advice and provision of unpublished data. Y. M. is supported by a scholarship from the Algerian Ministry of Higher Education and Scientific Research.
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Meier-Abt, F., Mokrab, Y. & Mizuguchi, K. Organic Anion Transporting Polypeptides of the OATP/SLCO Superfamily: Identification of New Members in Nonmammalian Species, Comparative Modeling and a Potential Transport Mode. J Membrane Biol 208, 213–227 (2006). https://doi.org/10.1007/s00232-005-7004-x
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DOI: https://doi.org/10.1007/s00232-005-7004-x