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Inhibitory effects of L- and T-type calcium antagonists on contractions of human detrusor muscle

  • Pharmacodynamics
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Abstract

The inhibitory and relaxant effects of the L-type calcium antagonists nifedipine, nimodipine, verapamil and diltiazem, and of the T-type calcium antagonist mibefradil, on contractions of isolated human detrusor muscle were investigated. The tissue was obtained from 10 patients undergoing cystectomy due to bladder cancer. Effects of the calcium antagonists at different concentrations on the concentration-response curves for carbachol were investigated. Furthermore, concentration-relaxation curves were performed using potassium-precontracted muscle strips. All L-type calcium antagonists suppressed the mean concentration-response curve of carbachol significantly at a concentration of 10−6 M. Mibefradil up to 10−5 M did not significantly suppress it. Nifedipine significantly reduced the carbachol-induced maximum contraction to 75% and 44%, verapamil to 75% and 67% of the appropriate control value at concentrations of 10−7 and 10−6 M, respectively. Diltiazem reduced it insignificantly to 96% and 71% at the above-mentioned concentrations. The concentration-relaxation experiments revealed following pD2-values and maximum relaxations of nifedipine, nimodipine, verapamil and diltiazem, respectively: 6.23, 6.37, 5.66, 5.81 and 85%, 83%, 82%, 90%. Maximum relaxations and pD2-values were not significantly different from each other. The lowest concentration, for which a significant effect compared to control in Student`s t-test was found, amounted to 10−10 M, 10−9 M, 10−7 M, 10−6.5 M and 10−4 M for nimodipine, nifedipine, diltiazem, verapamil and mibefradil, respectively. L-type calcium antagonists are very potent relaxant agents of the human detrusor muscle in vitro.

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Acknowledgements

We are grateful to Prof. Peter Alken and Dr. Maurice Stephan Michel (Mannheim, Germany) for giving the opportunity to do research. We are grateful to Mr. Joachim Brade (Mannheim, Germany) for statistical advice. This work was supported by the Juniorforschungsfond of the Faculty of Clinical Medicine Mannheim, Germany.

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  1. Department of Urology, University Hospital Mannheim, Theodor-Kutzer-Ufer 1-3, 68167, Mannheim, Germany

    Jasmin Katrin Badawi, Heng Li, Sigrun Langbein, Sun-Tscheol Kwon, Stefan Kamp & Stephan Bross

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  1. Jasmin Katrin Badawi
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Correspondence to Jasmin Katrin Badawi.

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Badawi, J.K., Li, H., Langbein, S. et al. Inhibitory effects of L- and T-type calcium antagonists on contractions of human detrusor muscle. Eur J Clin Pharmacol 62, 347–354 (2006). https://doi.org/10.1007/s00228-006-0100-8

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  • DOI: https://doi.org/10.1007/s00228-006-0100-8

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