Freshening rather than warming drives trematode transmission from periwinkles to mussels

Abstract

In the Western Baltic Sea, climate change is happening at much faster rate than in most other seas and organisms are additionally exposed to a steep and variable salinity gradient. Climate change has previously been shown to affect parasite transmission in other marine ecosystems, yet little is known about potential effects of warming and desalination on parasite–host interactions. In laboratory experiments, we determined the combined effects of projected seawater warming and freshening on the emergence, activity, survival, and infectivity of cercariae (free-swimming infectious stage) of the trematode Himasthla elongata (Mehlis 1831), shed from its first intermediate host, the periwinkle Littorina littorea (Linnaeus 1758), in the Baltic Sea. We also assessed the susceptibility of the second intermediate host, the mussel Mytilus edulis Linnaeus, 1758, to cercarial infections. Generally, salinity was the main driver, particularly of cercarial activity, infectivity, and mussel susceptibility to infection. At the lowest salinity (13), cercariae were 50% less active compared to the highest salinity (19). Infection success and host susceptibility followed a similar pattern, with 47% and 43% less metacercariae (encysted stage) present at salinity 13 than at salinity 19, respectively. In contrast, effects of simulated warming were found only for cercarial survival, with cercarial longevity being higher at 19 than at 23 °C. No significant interactions between temperature and salinity were found. In contrast to the literature, the results suggest that a climate change-driven freshening (partly also warming) may lead to a general decline of marine trematodes, with possible beneficial effects for the involved hosts.

Introduction

Climate change is expected to affect a wide range of interactions among marine organisms, including interactions between parasites and their hosts (Poulin and Mouritsen 2006; Marcogliese 2008). In general, parasites are widespread in aquatic environments, and often interact with various ecosystem stressors, both at the population and the community level of the hosts they infect (Sures 2008; Nachev and Sures 2015; Vidal-Martínez and Wunderlich 2017). At the same time, parasites have been shown to have profound impacts on aquatic ecosystems by numerous direct and indirect effects on host populations and communities (Mouritsen and Poulin 2009; Sures et al. 2017). For instance, as reported by Wood et al. (2007), grazing activity of infected Littorina sp. on macroalgae decreased by 40% compared to non-infected individuals, indirectly causing changes in community structure of ephemeral algae. Given the important ecological roles of parasitism, an understanding of the links between parasite infection levels in hosts and changing environmental conditions is crucial to identify the effects of climate change on parasites and their hosts in marine ecosystems.

Many parasites have complex life cycles, often involving several sequential hosts. The free-living transmission stages in between hosts are known to be particularly sensitive to abiotic factors (Pietrock and Marcogliese 2003; Koprivnikar et al. 2010). Of those factors, temperature has been studied most intensively (Marcogliese 2016), in particular in cercarial life cycle stages of trematodes. Cercarial stages emerge from a first intermediate gastropod host and penetrate the second intermediate host tissue, in which they transform into the following life cycle stage (metacercariae), awaiting ingestion by the final host (marine bird). Diverse experimental studies have demonstrated positive correlations between increasing seawater temperature and cercarial emergence and infectivity (Thieltges and Rick 2006; Koprivnikar and Poulin 2009; Studer and Poulin 2013). Both emergence and infectivity occasionally decrease after a certain threshold depending on the trematode species studied (McCarthy 1999; Thieltges and Rick 2006; Koprivnikar and Poulin 2009). Temperature can also affect transmission to the next host, by direct effects on the infectivity of free-living cercariae (Pechenik and Fried 1995; Studer et al. 2010) or changes in the susceptibility of the second intermediate host to parasite infection (Laverty et al. 2017).

Considering that global change is a complex of different interacting changes, abiotic factors, such as salinity or pH changes, other than temperature may further modify the emergence, survival, and infectivity of free-living stages of trematodes, as well as the resistance of their hosts (Zander 1998; Mouritsen 2002; Pietrock and Marcogliese 2003; Leiva et al. 2019). Moreover, previous comparative analyses (Morley 2011; Morley and Lewis 2013; Marcogliese 2016) confirmed a large range of thermal tolerance beyond optimum temperatures for cercarial emergence and survival. These findings suggest that temperature may not represent the most relevant variable for parasitism as thought previously. In coastal marine environments, salinity can play a prominent role in parasite transmission and previous studies found decreases in cercarial emergence and infectivity at reduced salinities (Lei and Poulin 2011). Salinity may interact with temperature as indicated by multi-factorial experiments. For example, Koprivnikar et al. (2010) found mean survival of cercarial stages of Acanthoparyphium spinulosum compromised at 35 and 40 at 25 °C but not at 20 °C. However, many of these multi-factorial experiments focused on single phases of cercarial life cycles only, not including other possibly relevant transmission and developmental steps.

In the Baltic Sea, salinity gradients represent one of the major drivers of benthic community composition (Ojaveer et al. 2010; Jasper et al. 2011; Vuorinen et al. 2015). The inflow of high-saline bottom water from the North Sea and the outflow of shallow low-saline water, mostly from river-runoff and precipitation (Müller et al. 2016), create a strong Baltic-wide salinity gradient from about 30 (Skagerrak and Kattegat region in the west, southwest) down to 3 in the northeast area of the Gulf of Bothnia. The steepest salinity gradient is found in the South-Western Baltic Sea (i.e., eastern coast of Denmark and northeastern German coast), where salinity drops from 20 to 8 over 400 km (Bonsdorff 2006). Due to this strong gradient of osmotic stress its land-locked nature, intense anthropogenic pressures, and its strong environmental fluctuations, the Baltic Sea represents a hot spot to study climate change effects. Regional impacts are accompanied by global changes such as warming and ocean acidification. The Baltic Sea was indeed identified as the fastest-warming sea worldwide, and one of the fastest under increased nutrient load and oxygen depletion in bottom waters (with the Black Sea and the East China Sea; Reusch et al. 2018).

In this study, we investigated the single and combined effects of seawater warming and freshening on different life stages of the trematode Himasthla elongata, one of the dominant trematode species in the Western Baltic Sea (Lauckner 1984a, b). This parasite uses marine snails (Littorina littorea) as first intermediate host and blue mussels (Mytilus edulis), and other bivalve species, as second intermediate host. Coastal birds feeding on mussels represent the final host (Lauckner 1984a, b). Himasthla sp. cercariae normally survive up to a maximum of 2 days, with 100% survival rate within 24 h (de Montaudouin et al. 2016). Cercariae of H. elongata at 20 °C were found to maintain infectivity for around 20 h (Greve 1997). We assessed the different transmission steps from the first to the second intermediate host (Fig. 1): (i) cercarial emergence from periwinkles, (ii) cercarial activity and survival after emergence, (iii) cercarial infectivity in mussels, and (iv) susceptibility of mussels to cercarial infection. For (iii), cercariae were treated but not the mussels, whereas for (iv), mussels were treated but not the cercariae. With this study, we contribute to a better understanding of the effects of expected warming and freshening on parasite transmission success in a Baltic Sea host–parasite system.

Fig. 1

Life cycle of the trematode Himasthla elongata and transmission steps investigated in this study: Exp. I: cercarial emergence from the first intermediate host, the periwinkle Littorina littorea; Exp. II: cercarial activity/survival; Exp. III: cercarial infectivity; Exp. IV: susceptibility of the second intermediate host, the blue mussel Mytilus edulis, to cercariae infection. Different colours represent the different phases of the life cycle on which we focused: the first host/cercarial phase (red), the cercarial free-living phase (yellow), and the phase within the metacercarial stage in the second intermediate host (blue)

Methods

Parasite and host collection

Approximately 500 periwinkles (L. littorea) were haphazardly collected by hand at Årøsund, Denmark (55.25°N, 9.70°E) in August 2017, and transported to the GEOMAR laboratory in Kiel, Germany. Here, periwinkles were kept together in plastic containers of 25 L filled with seawater at 19 and 16 °C (i.e., the conditions at the site and time of sampling), aerated and fed ad libitum with Ulva lactuca and Fucus vesiculosus. On the day after collection (measurement 0), each periwinkle was individually placed in a petri dish containing 10 mL of seawater (19) and incubated under constant illumination underneath heating lamps at 25–27 °C for 4 h to promote cercarial emergence (e.g., Mouritsen 2002). Subsequently, petri dishes were checked under a stereomicroscope for the presence of cercariae. Periwinkles shown to be infected with H. elongata by this method were then kept in 10 L plastic containers in aerated seawater at 19and 16 °C (fed ad libitum with U. lactuca and F. vesiculosus).

Mussels (M. edulis) of 40–50 mm of shell length (i.e., 1–1.5 years of age) were collected from the ‘Kieler Meeresfarm’, a marine aquaculture facility in the inner Kiel Fjord (54.36°N, 10.16°E), as these are known to be free from trematode parasite infection. To confirm the uninfected status of mussels, 50 individuals were dissected immediately after collection and tissues (squeezed between two glass slides) were checked for parasites under a stereomicroscope. No infected mussels were found.

General experimental design and set-up

All experiments were conducted using temperature and salinity (fully crossed) as well as time (only for cercarial output) as fixed factors, and periwinkle/mussel identity nested within water bath as random factor (See Electronic Supplementary Material, ESM Fig.S1). Temperature levels applied were 19 and 23 °C. These two temperature levels were chosen based on representative summer temperatures in shallow Western Baltic Sea habitats (19 °C as shown for the past 15 years in Kiel Fjord; Pansch et al. 2018), and the expected increment by 2100 (+ 4 °C; IPCC Report 2019). Salinity levels applied were 13, 16, and 19. Following predictions for the Baltic Sea, salinity is expected to drop due to increasing precipitation rates and freshwater runoff (Vuorinen et al. 2015; Johnson et al. 2018). We, thus, chose two severity levels of desalination, i.e., reduction by 3 and 6, respectively, from 19 which is the average salinity of Årøsund, the site where L. littorea was collected. Furthermore, the activity of L. littorea was shown to considerably decrease at salinities below 13.5 (Rosenberg and Rosenberg 1972).

The experiments were conducted in six temperature-controlled water baths (50 × 50 × 50cm) located in a single climate chamber (16 °C). Cercarial motility and survival was assessed in a different room, using six smaller temperature-controlled water baths (8 L). Three of the six water baths were set at 23 and three at 19 °C, and each water bath included all salinity levels. There were (i) five independent containers (one periwinkle per container) per salinity level per water bath for the cercarial emergence phase, (ii) one 96-well plate (15 cercariae, one cercaria per well) per salinity level per water bath for the functional activity/survival phase, and (iii) four independent containers (one mussel per container) per salinity level per water bath for the infection success and susceptibility phases. For each salinity level, filtered Kiel Fjord seawater was used and either marine salt (Seequasal) or deionized water was added, until the desired salinity was reached. In each experiment, temperature and salinity were measured with a digital salinity meter (ProfiLine, Cond 3110, WTW), within the experimental jars every other day.

Exp. I cercarial emergence from periwinkles

Cercarial emergence was assessed by counting all cercariae that emerged from single periwinkles exposed to the different treatments. Infected periwinkles (20–25 mm length) were haphazardly assigned to the different treatment combinations. Periwinkles were placed individually into 250 mL glass jars filled with seawater at the different salinities. Water at the respective salinity conditions was prepared by adding salt (Seequasal) or DI water as necessary. After individually adding the snails, each jar was tightly closed with a lid preventing evaporation and provided with a small hole to allow oxygenation through a tube (from air pumps). Salinity and temperature were checked every other day before the water change, and only minor salinity increments were registered (+ 0.2–0.3). Five jars per salinity treatment were placed into each waterbath (three baths for each temperature level x five periwinkles/jars for each salinity level, i.e., 15 periwinkles in total per treatment combination), to acclimate periwinkles to the respective temperature and salinity levels. Periwinkles were fed daily ad libitum with U. lactuca and the water was exchanged every second day. Acclimation to the different temperatures and salinities lasted for 1 week, increasing/decreasing temperature and salinity by 1 °C and 1 psu per day, respectively.

To measure cercarial emergence, periwinkles were individually transferred from the jars into 50 mL Plexiglas beakers (Omnilab, ESM Fig. S2a), 1 day after the 7-day acclimation (measurement 1), 1 week after the start of exposure to the treatments (measurement 2), and 2 weeks after the start of exposure to the treatments (measurement 3), representing three sequential measurements for each individual periwinkle. Each incubation lasted 8 h. Each beaker was filled with 40 mL of seawater of the respective salinity and small amounts of U. lactuca were added. The beakers were covered with a net and incubated for 8 h in the same water baths from which the periwinkles originated (ESM Fig. S2b) At the end of the incubation period, the periwinkles were individually placed back into their 250 mL glass jars at treatment conditions, while the water from each beaker was transferred into 50 mL Falcon tubes. In addition, the beakers were immediately washed with 5 mL ethanol (99%), and the solution was also added to the Falcon tube to ensure the collection of all cercariae and their preservation for later counting. Samples were centrifuged for five minutes at 800 g, excess water was discarded, and cercariae at the bottom were poured into a petri dish for counting under a stereomicroscope (Nikon, SMZ1000). Periwinkles that did not shed any cercariae over all the three incubations were excluded from the analysis.

Exp. II cercarial activity and survival

Infected periwinkles were acclimated for 3 weeks under the different treatments (n = 10 individuals per treatment) and incubated as described above for 4 h to allow for cercarial release. At the start of the experiment, approximately 45 cercariae per treatment, obtained from the pooled cercariae released within four hours from the ten snails per treatment, were added individually to wells of three 96-well plates (n = 15 wells into three replicate 96-well plates per salinity; 45 wells in total). The amount of water (of the respective salinity levels of 13, 16, and 19) in each well was around 0.25 mL. The 96-well plates were tightly closed with the respective manufacturer’s lids throughout the 45 h period preventing evaporation, except when cercariae were examined for activity and survival. The 96-well plates were then exposed to the different temperature treatments placing them in the six thermobaths (three thermobaths for each temperature level x one 96-well plate for each salinity level = three 96-well plates per treatment combination). Activity and survival were then assessed by visual assessment under a stereomicroscope after 4, 6, 9, 18, 27, and 45 h. Cercariae were considered fully active when they were constantly swirling around (category: fully active). When cercariae were laying at the bottom of a well and not reacting after physical stimuli (through pipette’s tip), these were considered dead (category: dead), when still reacting, even if slowly, these were still considered alive (category: alive). This last category included “fully active”. Wells that accidentally received more than just one cercaria during the inoculation were excluded from the analysis.

Exp. III cercarial infectivity in mussels

The infectivity of H. elongata cercariae was investigated by counting metacercariae in M. edulis, after a standardized exposure to cercariae acclimated at different temperatures and salinities. Infected periwinkles were acclimated to the treatments for 4 weeks prior to the infection success assays. Mussels were collected 2 days prior to the start of the assays, kept in ambient salinity and temperature conditions and fed with Rhodomonas salina. A total of 72 mussels (n = 12 for each treatment combination) of 40–50 mm shell length, kept at 19 psu and 16 °C, were individually placed in 50 mL beakers at the respective temperatures and salinities, and exposed to a standardized number of cercariae released from the all periwinkles from the different treatment combinations. In each waterbath, four beakers per salinity combination were placed, i.e., a total of 12 beakers per bath. To obtain cercarial stages for the infection assays, periwinkles were individually placed in petri dishes at the different salinities and incubated for four hours under constant light (see above). After release, all cercariae (4–5 h old) of a given treatment combination were collected mixing the water from the different petri dishes into one single container, counted under the stereomicroscope and immediately added to the mussels. This accounted for genotypic and phenotypic variation among cercariae. Each of the 12 mussels of a given treatment combination was inoculated with 22 (13–19 °C, 16–19 °C and 19–23 °C), or 23 (19–19 °C) cercariae, except for the 16–23 °C treatment, in which only five mussels where infected with 20 cercariae each due to the low number of cercariae obtained from such pre-treated periwinkles. The 13–23 °C treatment was excluded from the analyses due to extremely low numbers of cercariae released under this particular treatment combination. The beakers with mussels were then returned to the water baths at the treatment temperature and incubated for 24 h. This incubation period was chosen to ensure cercarial encystation (de Montaudouin et al. 2016), but to also limit possible metabolic stress for the host, that could have affected the experiment. After the incubation, each mussel was collected and dissected, and H. elongata metacercariae were identified and counted under a stereomicroscope.

Exp. IV susceptibility of acclimated mussels to cercarial infection

The effects of temperature and salinity on the susceptibility of M. edulis to infection by H. elongata cercariae were assessed by acclimating mussels to the above-mentioned treatment combinations (salinity × temperature) for 1 week, before these were exposed to non-acclimated cercariae. In this experiment, cercariae did not receive any temperature/salinity treatment and only the mussels did (after acclimation), to specifically investigate a putative shift in susceptibility under the treatments by the second intermediate host. We assumed acclimation effects to be stronger in the (acclimated) hosts and less likely on the very short-term basis in the added (non-acclimated) cercariae. However, some potential confounding factors by cercariae very quickly acclimatised cannot be fully excluded. Cercariae were obtained as described above from periwinkles collected 2 days earlier from the sampling site at Årøsund, Denmark. The exposure to cercariae of each mussel (n = 12 mussels per treatment) was realized as described for Exp. II, but this time, the number of cercariae added to individual mussels was 54 (16–19 °C, 16–23 °C and 19–23 °C), 53 (13–19 °C), and 56 (19–19 °C). Beakers with mussels were incubated at the different treatments for 24 h. Mussels were then dissected and the number of metacercariae in each mussel (squeezed between two glass plates) was counted under a stereomicroscope.

Statistical analysis

Prior to analysis, the normality of data distribution was tested through Shapiro–Wilk tests (Shapiro and Wilk, 1965) and residual plots were checked visually. Non-parametric Generalised Linear Mixed Models (GLMMs), using the packages lm4 and MASS, were then applied to cercarial emergence, infectivity, and mussel susceptibility. Residual extraction, the marginal R², and conditional R² were used to reveal individual identity and thermobath effect, using the function r.squared GLMM (package MuMIn; Nakagawa and Schielzeth 2013). Individual identity nested within thermobath was then used as random factor in all the steps analysed with GLMM. The Akaike Information Criterion (AIC) was used to select the best GLMM model, from models including all fixed factors and reduced models (without temperature or salinity as fixed factors). Two-way ANOVA was instead used for activity and survival. All statistical analyses were performed using the software R 3.5.0 (R Development Core Team 2018).

For cercarial emergence, a negative binomial GLMM, using the lme4 package, was used to test for the effects of temperature, salinity, and sampling time (fixed factors) on the number of H. elongata cercarie (Exp. I). Negative binomial structure was chosen due to high over-dispersion and presence of zeros in the data.

For cercarial activity/survival (Exp. II), the analysis applied was selected to link Exp II with Exp. III. For this purpose, we accounted of the differential slope of decreasing activity/survival during these 24 h by considering the integral over time (integrated area), which should correspond to the (changing) infection pressure that the mussels underwent in the infection experiments. The average proportion of “fully active” and “alive” cercariae present in one 96-well plate was considered as one replicate. Proportions were calculated through the ‘survfit’ function (package survminer). The integral areas of both 0–24 h and 0–45 h intervals were calculated using the mean proportions of the three 96-well plates to fit the curves. Curves of activity were fitted using geometric model (Power Low family), and the curves of survival using logistic power model (Sigmoidal models) (software: CurveExpert Professional 2.6.5). To test the effects, two separated two-factorial ANOVA were applied for each interval. Integrated motility and survival at 24 h and 45 h were the dependent variables, and temperature and salinity were the independent variables. Post hoc tests [Tukey honestly significant difference (HSD)] were performed following ANOVA.

Cercarial infectivity in M. edulis (Exp. III) was tested by a GLMM fitted with a Poisson structure with the number of metacercariae found in mussel tissue as dependent variable, and temperature and salinity were set as fixed factors. The same methodology as for infectivity was applied for susceptibility, however, here applying a GLMM fitted with a negative binomial structure.

Using the raw data, the effects of i) strong future freshening (salinity 13) in relation to present conditions (salinity 19) and ii) strong warming (23 °C) in relation to present condition (19 °C) were expressed as logarithmic response ratios (Lajeunesse 2015). For cercarial activity, only the 0–24 h interval was re-considered, linking this period to the infectivity experiment, in which mussels were incubated for 24 h. Effects were considered significant when the confidence interval (CI) bar was not overlapping zero. Effect sizes (in percentages) of the different life cycle steps were calculated applying the inverse of the log mean ratio for (i), all temperature levels were pooled, and for (ii), all salinity levels were pooled.

Results

Exp. I cercarial emergence

For cercarial emergence, model selection through AIC revealed four top models having ΔAICs of ≤ 2.1, with models including temperature or salinity and time, or an interaction between both (ESM Table S1). However, the model including only time as fixed factor described the data best (ESM Table S2). Cercariae output significantly decreased by 31% from the first to the second incubation in all treatments [GLMM, estimate value (ev) =  − 0.78, z =  − 2.65, P = 0.007, ESM Table S2], and by 49% from the first to the third incubation (GLMM, ev =  − 1.26, z =  − 3.92, P < 0.0001, ESM Table S2). However, this happened with clearly variable patterns among treatments. The strongest decrease of cercarial release over time was observed at salinity 13, while at salinity 19, it remained fairly constant throughout the 2 weeks (Fig. 2). At 13–19 °C only, the 50% of the periwinkles shed cercariae, compared to 70–80% as observed in the other treatments (ESM Fig. S3).

Fig. 2

Effects of temperature, salinity, and time on Himasthla elongata cercarial emergence during 8 h from Littorina littorea at three measurement points: a 1 day after acclimation, b 1 week after acclimation, and c 2 weeks after acclimation week in 19 and 23 °C seawater conditions at salinities of 13, 16, and 19. Each n indicates the number of periwinkles shedding cercariae in each treatment. The boxes represent the 75 (upper) and 25 (lower) percentile, the whiskers denote the lowest and the highest value, the black lines within the boxes the medians, the black diamond-shaped dots identify the means, and the black dots the outliers. Periwinkles that did not shed any cercariae in all the three incubations were excluded from the analysis and the plots

Exp. II cercarial activity and survival

In the integrated 0–24 h and 0–45 h, cercarial activity was significantly decreasing with salinity (ANOVA, 0–24 h: F_2,12 = 0.10, P = 0.001; 0–45 h: F_2,12 = 12.45, P = 0.001, Table 1). Cercarial activity over 24 h was 31% lower at salinity 16 than salinity 19 and 37% lower at salinity 13 than 19 (Tukey test 16–19: P = 0.004; salinities 13–19: P = 0.002). Cercarial activity over 45 h was 36% lower at salinity 16 than salinity 19 and 42% lower at salinity 13 than salinity 19 (Fig. 3; Tukey test: 16–19: P = 0.003; 13–19: P = 0.002). Cercarial survival showed no significant effects in the integrated 0–24 h interval. Contrarily, temperature had a negative effect in the integrated 0–45 h, with survival at 23 °C 19% lower than at 19 °C (ANOVA, F_1,12 = 32.12, P = 0.001, Table 1).

Table 1 Results of a two-way ANOVA testing for the single and interactive effects of temperature (19 and 23 °C) and salinity (13, 16, and 19) on the integrated 24 h and 45 h activity and survival of Himasthla elongata cercariae

Fig. 3

Proportion of Himasthla elongata cercariae considered as a fully active (i.e., constantly swirling around; and b alive over 2, 4, 6, 9, 18, 27, and 45 h post-release in 19 and 23 °C seawater conditions at salinities of 13, 16, and 19

Exp. III cercarial infectivity

Model selection indicated the model which only included salinity to be the best model (ESM Table S3). Infectivity significantly decreased with decreasing salinity. At salinity 16, the proportion of cercariae successfully infecting mussels was 33% lower than at salinity 19 (GLMM, ev = 0.46, z = 2.03, P = 0.041, ESM Table S4), and at salinity 13, this trait was 47% lower than at salinity 19 (GLMM, ev = 0.65, z = 2.97, P = 0.002, Fig. 4, ESM Table S4). Models including temperature as an additional additive or interactive factor, even if not significant, also received substantial support from the data (ΔAIC ≤ 2; ESM Table S3). While at salinity 19, infectivity was 21% higher at 23 °C compared to 19 °C, it was the opposite at salinity 16 (at 23 °C infectivity 44% lower than 19 °C) (Fig. 4). Only very few cercariae were shed from periwinkles at the 13–23 °C treatment. Therefore, infection success could not be tested for 13–23 °C.

Fig. 4

Proportion of acclimated cercariae successfully infecting non-acclimated Mytilus edulis (n = 12 for each treatment, apart from 16 to 23 °C, where n = 5) following a 24 h incubation in 19 and 23 °C seawater conditions at salinities of 13, 16, and 19. The 13–23 °C treatment was excluded from the experimental analyses due to the very low number of cercariae shed from the snails acclimatised to this respective treatment. The boxes represent the 75 (upper) and 25 (lower) percentile, the whiskers denote the lowest and the highest value, the black lines in the boxes are the medians, the black diamond-shaped dots identify the means, and the black dots the outliers

Exp. IV susceptibility of mussels

Model selection identified the model including only salinity as the best model (ESM Table S5). The proportion of cercariae infecting acclimatised mussels decreased significantly with salinity. At salinity 16, the proportion was 19% lower than salinity 19 (GLMM, ev = 0.32, z = 1.59, P = 0.110, ESM Table S6), and at salinity 13, it was 43% significantly lower than salinity 19 (GLMM, ev = 0.61, z = 3.03, P = 0.002, ESM Table S6). The model including temperature and salinity also explained the data reasonably well (ΔAIC 1.85, ESM Table S5). The patterns in the plot (Fig. 5) show a similar susceptibility between hosts at salinity 16 and at salinity 19 at 19 and 23 °C, but lower susceptibility at 23 °C than at 19 °C at salinity 13.

Fig. 5

Proportion of non-acclimated cercariae infecting acclimated Mytilus edulis (n = 12 for each treatment) following a 24 h incubation in 19 and 23 °C seawater conditions at salinities of 13, 16, and 19. The boxes represent the 75 (upper) and 25 (lower) percentile, the whiskers denote the lowest and the highest value, the black lines within the boxes the medians, and black diamond-shaped dots identify instead of the means

Future versus present conditions in our study system

After pooling the temperature levels, log response ratios showed a significant decrease of cercarial emergence (to 54%), cercarial activity (to 80%), cercarial infectivity (to 70%), and second intermediate host susceptibility (75%) in an extreme future freshening scenario from salinity 19 (ambient condition) to 13 (future condition; Fig. 6a). Contrarily, after pooling the salinity levels, and comparing future global warming (23 °C) with today’s temperatures (19 °C), only cercarial emergence and long-term survival (45 h) decreased significantly, to 70% and 90%, respectively (i.e., confidence intervals not overlapping zero; Fig. 6b).

Fig. 6

Logarithmic response ratios for: cercarial emergence, cercarial activity after 24 h, cercarial survival after 24 and 45 h, cercarial infectivity, and mussel (second intermediate host) susceptibility. a Effects of desalination (log [(trait at 13)/ (trait at 19)]) with data from the two temperature treatments pooled, and b effects of warming (log [(trait at 19 °C)/ (trait at 23 °C)]) with data from the three salinity levels pooled. Error bars represent 95% confidence intervals. Significant effects are indicated by ‘*’. Different colours represent the different effects on: (i) cercarial shedding from periwinkles (red), (ii) cercarial survival and infectivity (yellow), and (iii) susceptibility of mussel to infections (blue)

Discussion

To date, studies of environmental effects on the free-living stages of parasites in the Baltic Sea are rare, apart from experiments by Möller (1978) about the combined influence of temperature and salinity on the survival of fish parasites. In this study, we assessed the possible impact of warming and freshening on specific transmission stages from the first to the second intermediate host of a common marine trematode of the western Baltic Sea. Salinity had the strongest effects on most transmission steps: cercarial emergence, activity, infectivity, and second intermediate host susceptibility all decreased with decreasing salinity. In contrast, only cercarial emergence and survival were affected by temperature, both decreasing with the warming scenario. Interaction effects between salinity and temperature were weak or absent.

Cercarial emergence

Cercarial emergence decreased over the experimental duration; accordingly, time was the factor contributing to the model that fitted the data best. However, over the entire experimental period, cercarial emergence significantly decreased to 54% with sea water freshening (salinity 13) and to 70% with warming compared to the ambient condition (salinity 19; Fig. 6). Both observations are in line with the previous studies which showed a positive relationship between cercarial emergence and increase in salinity (Sindermann and Farrin 1962; Lei and Poulin 2011; Studer and Poulin 2012) as well as increase in temperature (Mouritsen 2002; Studer et al. 2010). Cercarial emergence typically increases with temperatures until an optimum, usually determined by the host tolerance and by acclimation to changed environmental conditions (Erasmus 1972; Ataev 1991; Morley and Lewis 2013). The number of cercariae released during our measurement 1 (after 7 days of acclimation) was relatively high compared to the next two incubations, especially at the lowest salinity (13). Here, the number of cercariae released was 35% higher than at ambient condition (salinity 19). This could hint at an initial response of periwinkles to the abrupt changes in salinity/temperature, which then possibly decreased with time. Hence, high fluctuation of environmental factors characterising the Western Baltic shallow coast (Franz et al. 2019) might favour a higher tolerance to stressors by benthic organisms. This is especially valid for L. littorea, which has been shown coping well with temperature peaks in a study by Pansch et al. (2018). The substantial variance of the responses within the treatment combinations could be also attributable to within-host and within-parasite population variance of sensitivities (Al Janabi et al. 2016; Leiva et al. 2019). Finally, since Littorina sp. inhabits shallow coasts, other factors could influence cercarial emergence, such as water level changes, as reported by Leiva et al. (2019). Further experiments applying frequent peaks of temperature and salinity changes over a short period, and accompanied by sea-level changes, would be valuable to investigate whether cercarial emergence is reacting stronger to short-term triggers than to long-term acclimations.

Cercarial activity and survival

Cercarial activity decreased significantly with freshening of seawater to 80% of the activity at ambient conditions (salinity 19; Fig. 6). Again, these salinity effects are consistent with the previous studies from other marine trematode species of the genus Acanthoparyphium (Echinostomatidae) and Maritrema (Microphallidae) and (Koprivnikar et al. 2010; Studer and Poulin 2012), generally showing cercariae to fare better with increasing salinity. The reason for the decreased cercarial activity at lower salinities is probably attributable to osmotic stress. Body fluids of periwinkles are known to be isosmotic down to 15, below which the fluids become hyperosmotic (Todd 1964; Rumsey 1973). Cercariae released from hosts under such low-salinity conditions might been subjected to an osmotic shock after emergence (Lauckner 1984a, b). Supporting this, in coastal waters with salinity below 15, H. elongata infections were registered only in periwinkles at the bottom of shallow waters (Lauckner 1984a, b). Stratification of the water column in the Baltic Sea often leads to the deeper water layer being higher in salinity and lower in temperature (Liblik and Lips 2019). In contrast to activity, cercarial survival was negatively affected by temperature only. Over 45 h, survival decreased significantly by 10% at the higher temperature (23 °C) (Fig. 6) compared to ambient (19 °C). Similar results have been reported previously (Mouritsen 2002; Koprivnikar et al. 2010), possibly resulting from higher temperatures accelerating the metabolism of cercariae leading to a faster depletion of their finite energy reserves (Pechenik and Fried 1995). This in turn might have direct consequences for their transmission success in a future warmed ocean.

Cercarial infectivity and susceptibility of mussels

Freshening had a negative effect on cercarial infectivity which decreased significantly with desalination to 70% compared to ambient condition (salinity 19; Fig. 6). Freshening has been shown previously to reduce cercarial infectivity, possibly because of the vulnerability of the free-living stages of marine trematodes to low salinity (Pietrock and Marcogliese 2003). Furthermore, as shown by Stunkard and Shaw (1931), osmotic stress could provoke slower cyst formation during infection. A previous study by Riisgård et al. (2013) found no differences in filtration rate of blue mussels collected at salinity 17.5 and exposed for 2 weeks to a range of salinities (10–30). Hence, the reduced infectivity with freshening could be merely attributed to the acclimatised cercariae. The susceptibility of the mussel host was also negatively affected by seawater freshening to 75% of the ambient condition (Fig. 6). Accounting that filtration rate at lower salinity is not significantly reduced during 1 week of acclimation, other reasons narrowed to susceptibility should be attributed, which remain unknown. However, the difference of 5% between decreased infectivity (70%) and susceptibility (75%) with desalination leads to argue that acclimation mattered. To exclude any effect of changes in filtration on susceptibility, a longer acclimation period to the mussels would be required. Moreover, further experiments investigating physiological responses of mussels while infected by cercariae could also be useful for a better understanding of mussel susceptibility. Interestingly, no significant effects of temperature or interaction between temperature and salinity were observed for both cercarial infectivity and mussel susceptibility. This suggests a limited importance of temperature for cercarial infectivity and mussel susceptibility in this parasite–host system. However, it may be that stronger warming scenario (> 4 °C) or extreme future conditions during heatwaves could have resulted in stronger temperature responses. For example, Pansch et al. (2018) found M. edulis to tolerate temperature peaks better than other bivalve species. Seemingly, only at very high temperatures, the mussel immune system may become compromised leading to an increase of cercarial infection.

Expected shifts in parasitism in response to desalination and warming

In general, our experiments suggest that future desalination scenarios for the Baltic Sea may lead to lower cercarial transmission rates, and therefore lower infection levels in mussel hosts, with salinity having more profound effects than temperature. Based on the combined effects of decreasing cercarial emergence (reduced to 54% with freshening), cercarial activity (reduced to 80% with freshening), cercarial survival (reduced to 90% with warming over 45 h), and mussel susceptibility (reduced to 75% with freshening), future climate scenarios could lead to a decrease in infection levels of H. elongata in mussels to around 30% (0.54 × 0.8 × 0.9 × 0.75). Such lower infection levels in mussels with decreasing salinity are also supported from field data along the Western Baltic coast, in which prevalence of trematodes in M. edulis at salinities below 15 was reduced by 80% relatively to higher salinities (Bommarito et al. unpublished.). These predictions suggest that global change may not always lead to elevated levels of parasitism as predicted by some studies (Poulin 2006; Studer et al. 2010), which could be beneficial for the host populations. It must be emphasized that the direction of global change effects is probably not universal, but conditional on the respective parasite–host systems, and the particular environmental context. Steep salinity gradients can be found in many coastal seas worldwide, for example, areas in which glacier meltwaters release freshwater into the sea, or the many estuaries resulting from larger river runoffs. Therefore, the data collected on the host-parasite system in the present study are not relevant to the Baltic Sea only, but might be applied to other host–parasite systems. Furthermore, H. elongata is distributed worldwide, and testing whether the response of this species to changes in temperature or salinity differs with latitude would be beneficial. Our experiments only investigated the transmission from the first to the second intermediate host. Further experiments on other life cycle stages of H. elongata, such as on eggs or miracidial stages, are needed to fully understand the climate change effects on this entire host–parasite system, and whether our findings can be extended to other marine trematode species. Acidification could be also included, especially in experiments involving molluscs as intermediate hosts. In addition, parasite transmission and host infection levels under climate change will also be affected by other factors such as the density of the intermediate hosts, the presence of the final hosts, as well as other environmental factors. All of these are important to consider in developing a full picture of the climate change effects on host-parasite interactions.

Ethical statement

This study was funded by the Ca’ Foscari University of Venice, Italy, and GEOMAR Helmholtz Centre for Ocean Research in Kiel, Germany, with no conflict of interest. All applicable international, national, and/or institutional guidelines for sampling, care, and experimental use of organisms for the study have been followed.