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Effect of alcohol-based organosolv treatment combined with short-time ball milling on the enzymatic hydrolysis of Japanese cypress (Chamaecyparis obtusa)

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Abstract

Japanese cypress (Chamaecyparis obtusa) is a recalcitrance softwood widely used as building material in Japan. A combined technique of alcohol-based organosolv treatment and ball milling (BM) of this softwood for the production of fermentable sugars from lignocellulose was investigated. Ethanol, ethylene glycol, water, and their mixtures were used as solvents, and acetic acid was used as a proton donor. After solvolysis, a residual sample was subjected to short-time BM, and the entire residue was evaluated by saccharification with Acremonium cellulase. Short-time BM after alcohol-based organosolv treatment greatly improved the enzymatic digestibility and decreased the required severity of organosolv treatment. Moreover, alcohol-based organosolv treatment increased the efficiency and reduced the time required for BM despite small quantity of removed lignin. It was found that the combination of alcohol-based organosolv treatment in mild condition and short-time BM had a synergistic effect on the enzymatic digestibility of Japanese cypress.

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Acknowledgments

This work was supported by a Grant-in-Aid for Scientific Research for the promotion and establishment of a recycling society from the Ministry of the Environment, Japan, the Shorai Foundation for Science and Technology, and by JSPS KAKENHI Grant Number 22780292. We thank Dr. Masahide Kawano and Mr. Muneaki Matsuda (Ehime University) for their useful comments. The author thank Meiji seika Co., Genencor Co., Novozyme Co., Shin Nihon Chemical Co., and Hankyu Bioindustry Co. for kindly supplying enzymes.

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Authors and Affiliations

  1. Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan

    Akihiro Hideno, Ayato Kawashima, Masayoshi Fukuoka, Katsuhisa Honda & Masatoshi Morita

  2. Biomass Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 737-0046, Japan

    Takashi Endo

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  1. Akihiro Hideno
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  2. Ayato Kawashima
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  3. Masayoshi Fukuoka
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  4. Takashi Endo
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  5. Katsuhisa Honda
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  6. Masatoshi Morita
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Correspondence to Masatoshi Morita.

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Hideno, A., Kawashima, A., Fukuoka, M. et al. Effect of alcohol-based organosolv treatment combined with short-time ball milling on the enzymatic hydrolysis of Japanese cypress (Chamaecyparis obtusa). Wood Sci Technol 47, 381–393 (2013). https://doi.org/10.1007/s00226-012-0504-9

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  • DOI: https://doi.org/10.1007/s00226-012-0504-9

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