Abstract
Medial meniscus posterior root tears (MMPRT) are often associated with osteoarthritis (OA) progression and subchondral bone insufficiency fractures. This study aimed to develop the first MMPRT mouse model. The MMPRT model was created by sectioning the medial meniscus posterior root of 12-week-old CL57BL/6J male mice under stereomicroscopic observation. The sham operation and the destabilization of the medial meniscus (DMM) model groups were also created. OA progression and subchondral bone changes were evaluated histologically using the Osteoarthritis Research Society International (OARSI) subchondral bone scoring system at 2, 4, 8, and 12 weeks after surgery. Microcomputed tomography (µCT) was performed to evaluate the presence of insufficient fractures. OA progression and medial meniscus extrusion were observed in the MMPRT and DMM models 12 weeks after surgery. OA progressed in both models during the time course, without a significant difference in the OARSI score between the two groups. The subchondral bone score was significantly higher at 12 weeks than at 2 and 4 weeks in the MMPRT group, while no significant difference was found between the two groups. In the µCT analysis, destruction of the medial tibial plateau was observed in 4/40 knees, while none were observed in the DMM group. Of the four knees, destruction of the medial femoral condyle was also observed in three knees. Characteristic pathological changes were observed in the mouse MMPRT model. The mouse MMPRT model may be useful for investigating pathological changes after MMPRT.
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Acknowledgements
We thank Ms. Minako Nagata, Ms. Maya Yasuda, and Ms. KyokoTanaka for their expert technical assistance. We would like to thank Editage (www. editage.com) for English language editing.
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Koji Nukuto, Takehiko Matsushita, Kohei Kamada, Kyohei Nishida, Kanto Nagai, Noriyuki Kanzaki, Yuichi Hoshino, Tomoyuki Matsumoto, Takahiro Niikura, Ryosuke Kuroda have no conflicts of interest in the authorship and publication of this article.
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All procedures were approved by the Institutional Animal Care and Use Committee of Kobe University Graduate School of Medicine (approval number P200410). No human data were used in this study.
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223_2022_1028_MOESM1_ESM.tif
Supplementary file1 (TIF 2700 KB) Supplemental Fig. 1 Sample allocation and collection in the medial meniscus root tear (MMPRT) model. Excld. Excluded from the analysis. PD patellar dislocation, NPD no patellar dislocation, FX Fracture, NFX no fracture
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Nukuto, K., Matsushita, T., Kamada, K. et al. Development and Analysis of Mouse Medial Meniscus Posterior Root Tear Model. Calcif Tissue Int 112, 55–65 (2023). https://doi.org/10.1007/s00223-022-01028-1
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DOI: https://doi.org/10.1007/s00223-022-01028-1