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Phases of intermediate filament composition in Bergmann glia following cerebellar injury in adult rat

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Abstract

In contrast to other astroglial populations, Bergmann glia (BG) form a strictly arranged system where each cell contacts the pia, with an architecture and function resembling that of immature radial glia. As a consequence, a post-lesion glial reaction is expected to differ from that observed in other parts of the brain. The present study describes the characteristic phases of intermediate filament protein formation during the different stages of BG response following injury and compares them with reactive glial patterns of other brain areas and patterns of glial development. The progress of Bergmann glial repair shares similar features with glial development. Following injury, BG developed nestin immunopositivity; then, colocalization of nestin and GFAP was observed. Finally, exclusively GFAP-immunopositive BG were restituted, denser, and thicker than before. The changes of intermediate filament composition appeared at first at the proximal and distal ends of BG fibers, i.e., at the perikaryal “root” and in the pial endfeet. No astrocytic invasion was present in the molecular layer, nor any distinct rearrangement of BG. These results demonstrate the role of the resident glia in glial reactions and refer to the priority of gliomeningeal connections.

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Acknowledgments

This work was supported by the Department of Anatomy, Histology and Embryology. We thank A. Őz, Zs. Vidra, Sz. Deák, and Zs. Feher for their technical assistance and E. Oszwald for her assistance with triple immunofluorescence. We gratefully acknowledge Dr. Sz. Mezey for the gift calbindin antibody and Dr. K. Kis-Petik for her help using FIJI program. Dr DS. Veres has greatly contributed to the statistical interpretation of our work. We thank Dr. J. Takács and Dr. RG Walker for their comments on the manuscript and A. Bárány for her assistance in the literature.

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Authors and Affiliations

  1. Department of Anatomy, Histology and Embryology, Semmelweis University of Medicine, Tuzolto 58, Budapest, 1094, Hungary

    Istvan Adorjan, Kinga Bindics, Peter Galgoczy & Mihaly Kalman

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  1. Istvan Adorjan
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  2. Kinga Bindics
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  3. Peter Galgoczy
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  4. Mihaly Kalman
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Correspondence to Istvan Adorjan.

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221_2014_3900_MOESM1_ESM.jpg

The cerebellar cortex on the seventh postoperative day following a coarse lesion. The immunopattern of the BG depends on the distance from the lesion track (L). Rectangle marks territory enlarged in Fig. 2g. (JPEG 495 kb)

221_2014_3900_MOESM2_ESM.jpg

The cerebellar cortex on the seventh postoperative day following a coarse lesion. The immunopattern of the BG depends on the distance from the lesion track (L). Rectangle marks territory enlarged in Fig. 2i (JPEG 159 kb)

221_2014_3900_MOESM3_ESM.jpg

The cerebellar cortex on the 30th postoperative day. GFAP immunopositivity was prevailing in BG (arrows) situated in the molecular layer (ML) and PCL of the tissue repair (asterisk). Nestin was restricted mainly to the mid-segments of BG (arrowheads). Purkinje cells (double arrowheads) were found only in the distant zone (DZ) but not in the area of tissue repair (asterisk) (JPEG 682 kb)

221_2014_3900_MOESM4_ESM.avi

The three-dimensional reconstruction of the traumatic zone at POD2. Nestin is visualized in red, GFAP is displayed in green, and DAPI is shown in blue. The mid-segments of the BG processes were nestin and GFAP immunonegative. Only the perikarya, initial segments, and the endfeet of BG displayed nestin immunopositivity in the traumatic zone (AVI 4631 kb)

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Adorjan, I., Bindics, K., Galgoczy, P. et al. Phases of intermediate filament composition in Bergmann glia following cerebellar injury in adult rat. Exp Brain Res 232, 2095–2104 (2014). https://doi.org/10.1007/s00221-014-3900-6

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