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Thiopental sodium reduces glutamate extracellular levels in rat intact prefrontal cortex

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Abstract

To investigate the effect of thiopental sodium on glutamate extracellular levels in the prefrontal cortex (PFC) of rats, a microdialysis probe was inserted into the PFC, the perfusate was collected every 10 min throughout the experiment with thiopental sodium ip or perfused into the PFC locally. The concentrations of glutamate in the perfusate were determined by reversed-phase high performance liquid chromatography. Thiopental sodium 30 mg kg−1 ip significantly decreased glutamate levels in the perfusate after 10, 20, 30, and 40 min; glutamate levels in the perfusate were also decreased from 10 to 90 min after thiopental sodium 50 mg kg−1 ip. Thiopental sodium with concentrations of 30, 100, or 300 μM perfused into the PFC also decreased glutamate levels in the perfusate significantly. The results suggest that thiopental sodium decreases glutamate extracellular levels in rat intact PFC.

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Acknowledgements

This work was supported by grants from national Nature and Science foundation of People’s Republic of China (No. 39970715).

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Authors and Affiliations

  1. Department of Anesthesiology, Zhongda Hospital, Southeast University, 210000, Nanjing, Jiangsu Province, People’s Republic of China

    Hongliang Liu

  2. Department of Anesthesiology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, Hubei Province, People’s Republic of China

    Shanglong Yao

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  1. Hongliang Liu
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  2. Shanglong Yao
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Correspondence to Hongliang Liu.

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Liu, H., Yao, S. Thiopental sodium reduces glutamate extracellular levels in rat intact prefrontal cortex. Exp Brain Res 167, 666–669 (2005). https://doi.org/10.1007/s00221-005-0243-3

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  • DOI: https://doi.org/10.1007/s00221-005-0243-3

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