Summary
To investigate the effect of thiopental sodium on the release of glutamate and γ-aminobutyric acid (GABA) from synaptosomes in the prefrontal cortex, synaptosomes were male, the spontaneous release and the evoked release by 30 mmol/L KCl or 20 μmol/L veratridine of glutamate and GABA were performed under various concentrations of thiopental sodium (10–300 μmol/L), glutamate and GABA concentrations were determined by reversed-phase high-performance liquid chromatography. Our results showed that spontancous release and evoked relcase of glutamate were significantly inhibited by 30 μmol/L, 100 μmol/L and 300 μmol/L thiopental sodium, IC50 of thiopental sodium was 25.8±2. 3 μmol/L for the spontaneous release, 23.4±2.4 μmol/L for KCl-evoked release, and 24.3±1.8 μmol/L for veratridine-evoked release. But GABA spontaneous release and evoked release were unaffected. The study showed that thiopental sodium with clinically related concentrations could inhibit the release of glutamate, but had no effect on the release of GABA from rats prefrontal cortical synaptosomes.
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Rocha A F, Pereira A J, Coutinho F A B. N-methy-D-aspartate channel and consciousness: from signal coincidence to quantum computing. Progr Neurobiol, 2001, 64: 555
Han J S eds. Science principle of neurology. 2nd ed. Beijing: the Publishing House of Beijing Medical University (Chinese), 1999, 953–956.
Miao N, Nagao K, Lynch C I. Thiopental and methohexital depress Ca2+ entry into and glutamate release from cultured neurons. Anesthesiology, 1998, 88: 1643
Buggy D J, Nicol B, Rowbotham D Jet al. Effects of intravenous anesthetic agents on glutamate release: a role for GABAA receptor-mediated inhibition. Anesthesiology, 2000, 92: 1067
Mantz J, Lecharny J B, Laudenbach Vet al. Anesthetics affect the uptake but not the depolarization-evoked release of GABA in rat striatal synaptosomes. Anesthesiology, 1995, 82: 502
Lecharny J B, Salord F, Henzel Det al. Effects of thiopental, halothane and isoflurane on the calcium-dependent and—independent release of GABA from striatal synaptosomes in the rat. Brain Res, 1995, 670: 308
Xu S Y, Bian R L, Chen X. Methodology of Pharmacological Experiment. 3rd ed. Beijing: People’s Mdical Publishing House (Chinese), 2001, 773
Xu S Y, Bian R L, Chen X. Methodology of Pharmacological Experiment. 3rd ed. Beijing: People’s Medical Publishing House (Chinese), 2001, 524
Pashkov V N, Westphalen R I, Hemmings H C. General anesthetics do not affect release of the neuropeptide cholecystokinin from isolated rat cortical nerve terminals. Anesthesiology, 2002, 97: 1500
Chen S S, Duan S M, Wang J. Rp-HPLC determination of amino-acids as neuro-transmitters with o-phthalaldehyds pre-column derivatization. J Xuzhou Med Col (Chinese), 1995, 22: 210
Joo D T, Xiong Z G, MacDonald J Fet al. Blocker of glutamate receptors and barbiturate anesthesia: increased sensitivity to pentobarbital-induced anesthesia despite reduced inhibition of AMPA receptors in GluR2 null mutant mice. Anesthesiology, 1999, 91: 1329
Kochan L D, Churn S B, Omojokun Oet al. Status epiletticus results in an N-methy-D-aspartate receptor dependent inhibition of Ca2+/calmodulin dependent kinase II activity in the rat. Neuroscience, 2000, 95: 735
Carr D B, Sesack S R. Collosal terminals in the rat prefrontal cortex: synaptic targets and association with GABA-immunoreactive structure. Synapse, 1998, 29: 193
Kuroda M, Yokofujita J, Murakami K. An ultrastructural study of the neural circuit between the prefrontal cortex and the mediodorsal nucleus of the thalamus. Progr. Neurobiol, 1998, 54: 417
Galli T, Desce J M, Artand Fet al. Modulation of GABA release by alpha-amino-3-hydroxy-5-methy-lisoxazole-4-propinate and N-methy-D-aspartate receptors in matrix enriched areas of the rat striatum. Neuroscience, 1992, 50: 769
Veh R W, Lichtinghagen R, Sewing Set al. Immunochemical localization of five members of the Kv 1 channel subunits: contrasting subcellular locations and neuron-specific co-localizations in rat brain. Eur J Neurosci, 1995, 7: 2189
Pirot S, Godbout R, Mantz Jet al. Inhibitory effects of ventral tegmental area stimulation on the activity of prefrontal cortical neurons: evidence for the involvement of both dopaminergic and GABAergic components. Neuroscience, 1992, 49: 857
Franks N P, Lieb W R. Molecular and cellular mechanisms of general anaesthesia. Nature, 1994, 367: 607
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LIU Hongliang, male, born in 1975, Doctor in Charge
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Hongliang, L., Shanglong, Y. Effect of thiopental sodium on the release of glutamate and γ-aminobutyric acid from rats prefrontal cortical synaptosomes. Current Medical Science 24, 602–604 (2004). https://doi.org/10.1007/BF02911368
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DOI: https://doi.org/10.1007/BF02911368