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Immunoreactivity for calcium-binding proteins defines subregions of the vestibular nuclear complex of the cat

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Abstract

The vestibular nuclear complex (VNC) is classically divided into four nuclei on the basis of cytoarchitectonics. However, anatomical data on the distribution of afferents to the VNC and the distribution of cells of origin of different efferent pathways suggest a more complex internal organization. Immunoreactivity for calcium-binding proteins has proven useful in many areas of the brain for revealing structure not visible with cell, fiber or Golgi stains. We have looked at the VNC of the cat using immunoreactivity for the calcium-binding proteins calbindin, calretinin and parvalbumin. Immunoreactivity for calretinin revealed a small, intensely stained region of cell bodies and processes just beneath the fourth ventricle in the medial vestibular nucleus. A presumably homologous region has been described in rodents. The calretinin-immunoreactive cells in this region were also immunoreactive for choline acetyltransferase. Evidence from other studies suggests that the calretinin region contributes to pathways involved in eye movement modulation but not generation. There were focal dense regions of fibers immunoreactive to calbindin in the medial and inferior nuclei, with an especially dense region of label at the border of the medial nucleus and the nucleus prepositus hypoglossi. There is anatomical evidence that suggests that the likely source of these calbindin-immunoreactive fibers is the flocculus of the cerebellum. The distribution of calbindin-immunoreactive fibers in the lateral and superior nuclei was much more uniform. Immunoreactivity to parvalbumin was widespread in fibers distributed throughout the VNC. The results suggest that neurochemical techniques may help to reveal the internal complexity in VNC organization.

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Abbreviations

BC:

brachium conjunctivum

BP:

brachium pontis

CD:

dorsal cochlear nucleus

CGL:

cochlear granular layer

CUR:

cuneate nucleus, rostral division

CVA:

ventral cochlear nucleus

CX:

external cuneate nucleus

DMV:

dorsal motor nucleus of the vagus

FTG:

gigantocellular tegmental field of the reticular formation

FTL:

lateral tegmental field of the reticular formation

INT:

nucleus intercalatus

MLF:

medial longitudinal fasciculus

PT:

pyramidal tract

PH:

nucleus prepositus hypoglossi

RB:

restiform body

S:

solitary tract

SA:

stria acoustica

SM:

medial nucleus of the solitary tract

TB:

trapezoid body

VIN:

inferior vestibular nucleus

VLD:

lateral vestibular nucleus, dorsal division

VLV:

lateral vestibular nucleus, ventral division

VMN:

medial vestibular nucleus

VNC:

vestibular nuclear complex

VSL:

superior vestibular nucleus, lateral division

VSM:

superior vestibular nucleus, medial division

5P:

principal sensory trigeminal nucleus

5SM:

alaminar spinal trigeminal nucleus, magnocellular division

5ST:

spinal trigeminal tract

6:

sixth cranial nerve nucleus

7N:

seventh cranial nerve

7G:

genu of the seventh cranial nerve

8N:

eighth cranial nerve

12:

twelfth cranial nerve nucleus

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Acknowledgements

Supported by NIH grants R01 EY07342 and DC01559. We thank Dr. Wade Sigurdson for use of, and help with, the confocal microscope. We thank Dr. Enrico Mugnaini for use of the Nikon microscope and camera.

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  1. Department of Physiology and Biophysics, School of Medicine and Biomedical Sciences, University at Buffalo, 123 Sherman Hall, Buffalo, NY, 14214-3078, USA

    Joan S. Baizer

  2. Department of Physiology and Institute for Neuroscience, Physiology/Medical, Ward 5-071, M211, Northwestern University Medical School, 303 East Chicago Avenue, Chicago, IL, 60611-3008, USA

    James F. Baker

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Correspondence to Joan S. Baizer.

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Baizer, J.S., Baker, J.F. Immunoreactivity for calcium-binding proteins defines subregions of the vestibular nuclear complex of the cat. Exp Brain Res 164, 78–91 (2005). https://doi.org/10.1007/s00221-004-2211-8

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