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Simultaneous detection of thiabendazole and carbendazim in foods based on two-color upconversion and magnetic separation nanoparticles fluorescence immunoassay

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Abstract

A rapid and sensitive immunoassay for the simultaneous detection of thiabendazole (TBZ) and carbendazim (CBZ) was developed by using magnetic nanoparticles (MNPs) and two-color upconversion nanoparticles (UCNPs). The anti-thiabendazole and anti-carbendazim antibody were linked to NaYF4:Yb, Tm (emission at 474 nm) and NaYF4:Yb, Er (emission at 542 nm) UCNPs as multicolor signal probe, respectively. The thiabendazole and carbendazim coating antigen were connected to magnetic microspheres as capture probe, respectively. Based on a competitive format, capture probes compete with analytes to combine corresponding signal probes. Under excitation at 980 nm, the formed competitive immune-complex fluorescent signal at 474 and 542 nm indicates the concentration of thiabendazole and carbendazim, respectively. Under the optimal conditions, the 50% inhibiting concentration (IC50) and limit of detection (LOD, IC10) were 2.21 and 0.083 ng/mL for thiabendazole and 1.18 and 0.075 ng/mL for carbendazim, respectively. The average recoveries of thiabendazole and carbendazim in foods, including oyster mushroom, shiitake mushrooms, oranges and orange juice, ranged from 83.6 to 100.9% with the coefficient of variance (CV) of 1.0–9.8% for thiabendazole and ranged from 89.6 to 105.9% with the CV of 2.5 to 9.1% for carbendazim. This multiplexed analysis format has potential applications for rapid, efficient detection of multiple food risk factors.

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Schematic diagram of the detection scheme

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Funding

This study was supported by the Natural Science Foundation of Tianjin (18JCQNJC84400) and National Natural Science Foundation (No. 31301487).

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Author notes

  1. Minsi Wang and Wenbo Zhu contributed to the work equally and should be regarded as co-first authors.

Authors and Affiliations

  1. Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Sciences, Tianjin Normal University, Tianjin, China

    Minsi Wang, Wenbo Zhu, Lingyan Zhao, Jingyi Jin & Yang Song

  2. Technical Center for Safety of Industrial Products of Tianjin Customs District, Tianjin, 300308, China

    Ming Liu

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  1. Minsi Wang
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  2. Wenbo Zhu
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  3. Lingyan Zhao
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  4. Jingyi Jin
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Correspondence to Yang Song or Ming Liu.

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Wang, M., Zhu, W., Zhao, L. et al. Simultaneous detection of thiabendazole and carbendazim in foods based on two-color upconversion and magnetic separation nanoparticles fluorescence immunoassay. Eur Food Res Technol 248, 85–94 (2022). https://doi.org/10.1007/s00217-021-03853-4

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  • DOI: https://doi.org/10.1007/s00217-021-03853-4

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