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Evaluation of the proteolytic activity of Enterococcus faecalis FT132 and Lactobacillus paracasei FT700, isolated from dairy products in Brazil, using milk proteins as substrates

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Abstract

Lactic acid bacteria (LAB) have been used by mankind from immemorial times due to their technological properties and ability to improve sensorial properties of foods. Some LAB strains are also able to hydrolyze milk proteins, which increase their digestibility and contribute to the production of desirable flavors. Moreover, proteolytic activity of LAB on milk proteins may release bioactive peptides with different activities such as antihypertensive, antioxidant, antimicrobial, immunomodulatory and mineral-binding activities. This study aimed to evaluate the proteolytic activity of Enterococcus faecalis FT132 and Lactobacillus paracasei FT700, isolated from Brazilian dairy products. The proteolytic activity was checked by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and high-performance liquid chromatography using milk proteins as substrates in different conditions. Both E. faecalis FT132 and L. paracasei FT700 were proteolytic at pH 6.5 in the range of 37–42 °C, and these activities were due to metalloproteases. L. paracasei FT700 could then be used as adjunct culture in fermented dairy products in order to increase their digestibility. On the contrary, E. faecalis FT132, which harbors three virulence genes asa1, ace and gelE and which is resistant to erythromycin and tetracycline, cannot be added to food products. However, the peptides produced after hydrolytic activity on milk proteins by both strains might be used as ingredients in purified formulas.

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Acknowledgments

The authors thank the São Paulo Research Foundation (FAPESP) for fellowship to Fabrício Luiz Tulini (#2011/11983-0, #2012/11379-8), the National Council for Scientific and Technological Development (CNPq) for financial support (#480772/2011-8), and the Coordination for the Improvement of Higher Education Personnel (CAPES) for fellowship to Vanessa Bíscola (#9694/11-0). This study is part of the cooperation program CAPES-COFECUB 730/11.

Conflict of interest

F.L. Tulini, V. Bíscola, Y. Choiset, N. Hymery, G. Le Blay, E.C.P. De Martinis, J-M Chobert and T. Haertlé declare that they have no conflict of interest.

Compliance with ethical requirements

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/n, Monte Alegre, Ribeirão Prêto, São Paulo, 14040-903, Brazil

    Fabrício Luiz Tulini & Elaine Cristina Pereira De Martinis

  2. Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Avenida Professor Lineu Prestes, 580, Butantã Cidade Universitária, São Paulo, São Paulo, 05508-000, Brazil

    Vanessa Bíscola

  3. UR 1268, INRA, Biopolymers Interactions Assemblies, Nantes Cedex 3, 44316, France

    Vanessa Bíscola, Yvan Choiset, Jean-Marc Chobert & Thomas Haertlé

  4. UEB EA3882 Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, ESIAB, Université de Brest, Technopôle de Brest-Iroise, Plouzané, 29280, France

    Nolwenn Hymery & Gwenaelle Le Blay

  5. UEB, Institut Universitaire Européen de la Mer (IUEM), UMR UBO, CNRS, IFREMER 6197, Laboratoire de Microbiologie des Environnements Extrêmes (LMEE), Université de Brest, Place Nicolas Copernic, Plouzané, France

    Gwenaelle Le Blay

Authors
  1. Fabrício Luiz Tulini
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  2. Vanessa Bíscola
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  3. Yvan Choiset
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  4. Nolwenn Hymery
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  5. Gwenaelle Le Blay
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  6. Elaine Cristina Pereira De Martinis
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  7. Jean-Marc Chobert
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  8. Thomas Haertlé
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Corresponding author

Correspondence to Elaine Cristina Pereira De Martinis.

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Tulini, F.L., Bíscola, V., Choiset, Y. et al. Evaluation of the proteolytic activity of Enterococcus faecalis FT132 and Lactobacillus paracasei FT700, isolated from dairy products in Brazil, using milk proteins as substrates. Eur Food Res Technol 241, 385–392 (2015). https://doi.org/10.1007/s00217-015-2470-6

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  • DOI: https://doi.org/10.1007/s00217-015-2470-6

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