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Comparative evaluation of ELISA kits’ reliability for the aflatoxin M1 determination in goat milk

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Abstract

Enzyme-linked immunosorbent assay (ELISA) is widely used in the food industry for detecting aflatoxin M1 (AFM1) in milk. The purpose of this survey was to compare the performance of three ELISA kits for the determination of AFM1 in goat milk by evaluating specific performance parameters such as precision, accuracy, repeatability and specificity, with AFM2 coexistence. Goat milk, with known AFM1 concentration (5 ng l−1), was spiked with AFM1 at 12.5, 25, 50, 75 and 100 ng l−1 and also with AFM2 in the same concentrations. At extremely low concentrations of AFM1 (17.5 ng l−1), all kits were imprecise, while by increasing the AFM1 concentration, at levels close to or higher than the maximum tolerable limit (55, 80 and 100 ng l−1) precision significantly improved for all kits. All kits presented high repeatability and fairly good specificity, regardless of the AFM2 presence that was examined for the first time.

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Acknowledgments

The authors wish to thank the three kit providers (ADAMS, BIOTICA, ATROPOS) for their kind kit donation and the National Reference Lab for Mycotoxins for collaborating in HPLC analysis. We are also grateful to DELTA Trofima, Xanthi’s Plant and Mr. Baltopoulos Vassilios for their valuable help and contribution providing us the sample.

Conflict of interest

None.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Postgraduate Course “Applied Public Health and Environmental Hygiene”, Faculty of Medicine, University of Thessaly, Papakyriazi 22, 41222, Larissa, Greece

    Sofia Christoforidou

  2. Laboratory of Hygiene and Epidemiology, Faculty of Medicine, University of Thessaly, Papakyriazi 22, 41222, Larissa, Greece

    Eleni Malissiova & Christos Hadjichristodoulou

  3. Department of Food Technology, Technological Educational Institution of Thessaly, Terma Temponera, 43100, Karditsa, Greece

    Eleni Malissiova & Olga Gortzi

Authors
  1. Sofia Christoforidou
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  2. Eleni Malissiova
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  3. Olga Gortzi
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  4. Christos Hadjichristodoulou
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Correspondence to Olga Gortzi.

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Christoforidou, S., Malissiova, E., Gortzi, O. et al. Comparative evaluation of ELISA kits’ reliability for the aflatoxin M1 determination in goat milk. Eur Food Res Technol 240, 701–706 (2015). https://doi.org/10.1007/s00217-014-2374-x

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  • DOI: https://doi.org/10.1007/s00217-014-2374-x

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