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Purification and characterization of a novel angiotensin I-converting enzyme inhibitory peptide derived from abalone (Haliotis discus hannai Ino) gonads

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Abstract

Abalone gonads were hydrolyzed using alcalase followed by papain to produce angiotensin I-converting enzyme (ACE) inhibitory peptides. Gel filtration column chromatography analysis showed that the molecular weight of the fraction with high ACE inhibitory activity was below 1 kDa, which occupied 93.1 % of total hydrolysate. The ACE inhibitory peptide was purified by a series of column chromatographies, and the sequence of purified peptide was further identified as Ala–Met–Asn (AMN) by automated Edman degradation method. The triple peptide was then synthesized and had an ACE inhibitory activity with IC50 value of 106.24 μg/mL. After gastrointestinal digestion, the peptide still remained bioactivity. Lineweaver–Burk plots indicated that AMN acts as a non-competitive inhibitor against ACE. Our present study suggested that AMN derived from abalone gonad by-products may be used as an ideal nutrient for development of functional foods.

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Acknowledgments

This study was sponsored by the National Natural Scientific Foundation of China (No. 31271838), the Key Project of the Ministry of Science and Technology of China (2012BAD38B09), the Public Science and Technology Research Fund Project of Ocean (201305015-3), and the Science and Technology Bureau of Xiamen (3502Z20133020).

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None.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. College of Food and Biological Engineering, Jimei University, Jimei, Xiamen, 361021, China

    Qiang Wu, Qiu-Feng Cai, Zhi-Peng Tao, Le-Chang Sun, Jian-Dong Shen, Ling-Jing Zhang, Guang-Ming Liu & Min-Jie Cao

  2. Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Xiamen, 361102, Fujian Province, China

    Qiang Wu, Qiu-Feng Cai, Zhi-Peng Tao, Le-Chang Sun, Jian-Dong Shen, Ling-Jing Zhang, Guang-Ming Liu & Min-Jie Cao

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  1. Qiang Wu
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Correspondence to Min-Jie Cao.

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Wu, Q., Cai, QF., Tao, ZP. et al. Purification and characterization of a novel angiotensin I-converting enzyme inhibitory peptide derived from abalone (Haliotis discus hannai Ino) gonads. Eur Food Res Technol 240, 137–145 (2015). https://doi.org/10.1007/s00217-014-2315-8

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  • DOI: https://doi.org/10.1007/s00217-014-2315-8

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