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Binding of a novel bacteriostatic agent—chitosan oligosaccharides–kojic acid graft copolymer to bovine serum albumin: spectroscopic and conformation investigations

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Abstract

In this paper, a novel chitosan oligosaccharides derivative—chitosan oligosaccharides–kojic acid graft copolymer (COS/KA), COS, and KA were selected to investigate the binding to bovine serum albumin (BSA) using UV–Vis spectroscopy, fluorescence, synchronous fluorescence, and circular dichroism (CD) at pH 7.4. The results indicated that the tryptophan residues of BSA to the distance of COS/KA, COS, and KA were <8 nm, the quenching constants K0 were decreased, and the value of Kq were much >2.0 × 1010 L mol−1 s−1 for COS/KA–BSA, COS–BSA, and KA–BSA at 298, 302, 306, 310 K, respectively, those confirmed that the quenching process belongs to static quenching. The thermodynamic parameters ∆H°, ΔG°, ΔS° at different temperatures were calculated and indicated that hydrophobic and electrostatic forces played important roles in the binding process, and the enhanced binding affinity mainly associated with the increase of the hydrophobicity. Moreover, the CD data demonstrated that the secondary structure of BSA was slightly altered in the presence of COS/KA, COS, and KA, with different reduced α-helix contents (46.30 ± 0.20, 48.60 ± 0.40, 50.70 ± 0.20), respectively. The work provides basic data for the binding mechanism of COS/KA with BSA in vitro.

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Acknowledgments

We thank the staff at our laboratory for the assistance they have provided in this study. This research was financially supported by the Twelfth Five Year National Science and Technology Plan of rural field research mission contract (2011BAD23B00), and the Fund Project for Transformation of Scientific and Technological Achievements of Jiangsu Province (BA2009082).

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None.

Compliance with Ethics Requirements

This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Lihu Road 1800, Wuxi, 214122, Jiangsu, People’s Republic of China

    Xiaoli Liu, Wenshui Xia, Qixing Jiang, Yanshun Xu & Peipei Yu

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  1. Xiaoli Liu
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  2. Wenshui Xia
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Correspondence to Wenshui Xia.

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Liu, X., Xia, W., Jiang, Q. et al. Binding of a novel bacteriostatic agent—chitosan oligosaccharides–kojic acid graft copolymer to bovine serum albumin: spectroscopic and conformation investigations. Eur Food Res Technol 240, 109–118 (2015). https://doi.org/10.1007/s00217-014-2312-y

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