Abstract
The potential of microsatellites in traceability of Tunisian oils was assessed by comparison of the genetic profile for six SSR markers of DNA extracted from oil and leaves of the two Tunisian major olive cultivars: Chemlali and Chetoui. Different protocols of DNA extraction from oil were tested and compared and we found that the most reproducible protocol in terms of successful microsatellites amplification was the Qiagen QIAamp DNA Stool extraction Kit, which we slightly modified. After amplification and automatic genotyping of six SSRs, it has been shown that the pattern of the DNA purified from a monovarietal oil did not correspond exactly to the profile of the same cultivar leaves’ DNA. In fact, we found evidence for the presence in the oil of alleles originating from the pollinators, which are present in the genome of the seed embryo. These alleles appear as minor peaks and could be easily distinguished from the mother tree’s alleles, so they do not interfere with oil traceability.
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References
Minelli S, Maggini F, Gelati MT, Angiolillo A, Cionini PG (2000) The chromosome compliment of Olea europaea L.: characterization by differential staining of the chromatin and in situ hybridization of highly repeated DNA sequences. Chromosome Res 8:615–619
Doveri S, Donal M, Lee D (2006) Non concordance between genetics profiles of olive oil and fruit: a cautionary note to the use of DNA markers for provenance testing. J Agric Food Chem 54(24):9221–9226
Besnard G, Khadari B, Villemur P, Bervillé A (2000) Cytoplasmic male sterility in the olive (Olea europaea L.). Theor Appl Genet 100:1018–1024
Diaz A, Martin A, Rallo P, Barranco D, de la Rosa R (2006) Self-incompatibility of ‘Arbequina’ and ‘Picual’ olive assessed by SSR markers. J Am Soc Hortic Sci 131:250–255
Roche HM, Gibney MJ, Kafatos A, Zampelas A, Williams CM (2000) Beneficial properties of olive oil. Food Res Int 33:227–231
Christopoulou E, Lazakari M, Komaitis M, Kaselimis K (2004) Effectiveness of determination of fatty acids and triglycerides for the detection of adulteration of olive oils with vegetable oils. Food Chem 84:463–474
Busconi M, Foroni C, Corradi M, Bongiorni C, Cattapan F, Fogher C (2003) DNA extraction from olive oil and its use in the identification of the production cultivar. Food Chem 83:127–134
Woolfe M, Primrose S (2004) Food forensic: using DNA technology to combat misdescription and fraud. Trends Biotechnol 22:222–226
Pafundo S, Agrimonti C, Marmiroli N (2005) Traceability of plant contribution in olive oil by amplified fragment length polymorphisms. J Agric Food Chem 53:6995–7002
Testolin R, Lain O (2005) DNA extraction from olive oil and PCR amplification of microsatellite markers. Food Sci 70:108–112
Muzzalupo I, Perri E (2002) Recovery and characterization of DNA from virgin olive oil. Eur Food Res Technol 214(6):528–531
De la Torre F, Bautista R, Cànovas FM, Claros G (2004) Isolation of DNA from olive oil and sediments: application in oil fingerprinting. Food Agric Envir 2(1):84–89
Jobling MA, Gill P (2004) Encoded evidence: DNA in forensic analysis. Nat Rev Genet 5:739–751
Rekik I, Salimonti A, Grati Kamoun N, Muzzalupo I, Lepais O, Gerber S, Perri E, Rebai A (2008) Characterization and identification of Tunisian olive tree varieties by microsatellite markers. Hort Sci 43(5):1371–1376
Sefc KM, Lopes MS, Mendoncxa D, Machado M, Da Camara Machado A (2000) Identification of microsatellite loci in olive (Olea europea) and their characterization in Italian and Iberian olive trees. Mol Ecol 9:1171–1173
Carriero F, Fontanazza G, Cellini F, Giorio G (2002) Identification of simple sequence repeats (SSRs) in olive (Olea europea L.). Theor Appl Genet 104:301–307
Cipriani G, Marrazzo MT, Marconi R, Cimato A, Testolin R (2002) Microsatellite markers isolated in olive (Olea europaea L.) are suitable for individual fingerprinting and reveal polymorphism within ancient cultivars. Theor Appl Genet 104:223–228
Muzzalupo I, Stefanizzi F, Perri E (2009) Molecular characterization and classification of olive (Olea europaea L.) tree genotypes geographically related in an Italian olive collection. Hort Sci (in press)
Taamalli W, Geuna F, Banfi R, Bassi D, Daoud D, Zarrouk M (2006) Agronomic and molecular analyses for the characterisation of accessions in Tunisian olive germplasm collections. J Biotechnol 9(5)
Ribeiro H, Abreu I, Cunha M, Mota T, Castro R (2005) Aeropalynological study of Vitis vinifera in Braga region (1999–2003). Aerobiologia 21:131–138
Acknowledgments
This work was supported by the Tunisian Ministry of Agriculture and the ADNid Company (Montpellier, France). We are particularly grateful to Jean-Fraçois Ballester, director of ADNid and to all its staff.
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Ayed, R.B., Grati-Kamoun, N., Moreau, F. et al. Comparative study of microsatellite profiles of DNA from oil and leaves of two Tunisian olive cultivars. Eur Food Res Technol 229, 757–762 (2009). https://doi.org/10.1007/s00217-009-1111-3
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DOI: https://doi.org/10.1007/s00217-009-1111-3