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Quantification of DNA during winemaking by fluorimetry and Vitis vinifera L.-specific quantitative PCR

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Abstract

The ability to genotype wine would be of a great benefit to control the varietal wine authenticity. In this paper, we assessed the fate of grapevine DNA and total DNA during vinification to suggest the latest sampling point that would allow genotyping. The total DNA concentration was measured by fluorimetry and the specific Vitis vinifera L. DNA concentration was measured by quantitative PCR in samples obtained from different steps of vinifications of four wines. To elucidate the effect of pre-extraction and extraction steps on DNA yield from wine, several samples from bottle of wine, two precipitation salts, and two DNA extraction solutions were tested. It was found that hundreds of genome equivalents of grapevine DNA can be extracted from every winemaking sample and from wine. In tested samples, differences in DNA yield among wines from different grapevine varieties, differences among extraction methods, and sampling variation in wine were not statistically significant.

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Abbreviations

CTAB:

Cetyltrimethylammonium bromide

GuSCN:

Guanidium isothiocyanate

qPCR:

Quantitative PCR

RAPD:

Random amplification of polymorphic DNAs

AFLP:

Amplified fragment length polymorphism

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Acknowledgment

This work was supported by grant of the Czech Ministry of Education 1K04104. Partial results of this project were presented at Ampelos 2006—2nd International Conference in Santorini, Greece. We thank Dr. M. Navrátil for generous instrumental provision.

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Authors and Affiliations

  1. Department of Biochemistry, Palacký University, Šlechtitelů 11, 78371 , Olomouc, Czech Republic

    Jiří Drábek, Michaela Jalůvková, Tomáš Jurček & Ivo Frébort

  2. Department of Post-Harvest Technology for Horticultural Products, Mendel University of Agriculture and Forestry Brno, 691 44, Lednice, Czech Republic

    Jan Stávek

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  1. Jiří Drábek
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  2. Jan Stávek
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  3. Michaela Jalůvková
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  4. Tomáš Jurček
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  5. Ivo Frébort
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Correspondence to Jiří Drábek.

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Drábek, J., Stávek, J., Jalůvková, M. et al. Quantification of DNA during winemaking by fluorimetry and Vitis vinifera L.-specific quantitative PCR. Eur Food Res Technol 226, 491–497 (2008). https://doi.org/10.1007/s00217-007-0561-8

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  • DOI: https://doi.org/10.1007/s00217-007-0561-8

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