Abstract
With established ampelographic techniques for grapevine identification it is often difficult to achieve a satisfactory, objective result. We have developed a DNA typing system using sequence-tagged microsatellite site markers as a means of differentiating cultivars of grapevine. A semi-automated analysis procedure was linked to an electronic database and found to be an objective and reliable system for cultivar identification using this simple marker type. The accumulated DNA typing data from over eighty cultivars demonstrated that cultivars that are difficult to differentiate phenotypically using ampelographic techniques can be distinguished by DNA typing. Parentage analysis uncovered errors in parent assignment of cultivar identification in specific cases. The electronic database has a conservative format to take into account the occurrence of null alleles and the possibility of missed alleles. Computer-assisted comparisons of cultivars in the database can be performed and various approaches for estimating the match probability that two unrelated cultivars have the same genotype simply due to chance are discused. We suggest that further development of the database through international co-operation using standardised sequence-tagged site markers offers the possibility of achieving a universal grapevine identification system.
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References
Alleweldt G: The genetic resources of Vitis. Federal Research Centre for Grape Breeding, Geilweilerhof, Germany (1988).
Alleweldt G, Spiegel-Roy P, Reisch B: Grapes (vitis). In: Moore JN, BallingtonJr JR (eds) Genetic Resources of Temperate Fruit and Nut Crops 1, pp. 289–327. International Society for Horticultural Science, Wageningen (1990).
Antcliff AJ: Variety identification in Australia. Australian Grapegrower and Winemaker 153: 10–13 (1976).
Callen DF, Thompson AD, Shen Y, Phillips HA, Richards RI, Mulley JC, Sutherland GR: Incidence and origin of null alleles in the (AC)n microsatellite markers. Am J Human Genet 52: 922–927 (1993).
Chakraborty R, Kidd KK: The utility of DNA typing in forensic work. Science 254: 1735–1739 (1991).
Committee on DNA Technology in Forensic Science, Board on Biology, Commission on Life Sciences, National Research Council: DNA technology in forensic science. National Academy Press, Washington, DC (1992).
Edwards A, Hammond HA, Jin L, Caskey CT, Chakraborty R: Genetic variation at five trimeric and tetrameric tandem repeat loci in fourhuman population groups. Genomics 12: 241–253 (1992).
Galet P: Cépages et Vignobles de France. P. Déhan, Montpellier (1956).
Galet P: A practical ampelography-grapevine identification. Commstock Publishing Association, Cornell University Press (1979).
International Board for Plant Genetic Resources:grape Descriptors. IBPGR Secretariat, Rome, AGPG:IBPGR/83/154 (1983).
Kwiatkowski DJ, Henske EP, Weimer K, Ozelius L, Gusella JF, Haines J: Construction of a GT polymorphism map of human 9q. Genomics 12: 229–240 (1992).
Lewontin RC, Hartl DL: Population genetics in forensic DNA typing. Science 254: 1745–1750 (1991).
Olson M, Hood L, Cantor D, Botstein D: A common language for physical mapping of the human genome. Science 245: 1434–1435 (1989).
Schlötterer C: Non-radioactive analysis of multiplexed microsatellite reactions using a direct blotting-sequencing apparatus. Nucl Acids Res 21: 780 (1993).
Tassie L: Ampelography — some updates. Australian Grapegrower and Winemaker 340: 66–71 (1992).
Thomas MR, Matsumoto S, Cain P, Scott NS: Repetitive DNA of grapevine: classes present and sequences suitable for cultivar identification. Theor Appl Genet 86: 173–180 (1993).
Thomas MR, Scott NS: Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequenced-tagged sites (STSs). Theor Appl Genet 86: 985–990 (1993).
Truel P, Rennes C, Domergue P: Identifications in collections of grapevines. In: Proceedings 3rd International Symposium on Grape Breeding, pp. 78–86. Dept. of Viticulture and Enology University of California, Davis, CA (1980).
Walker MA, Boursiquot JM: Ampelographic and isozyme data correcting the misnaming of the grape rootstock SO4 at the University of California, Davis. Am J Enol Vitic 43: 261–265 (1992).
Weber JL: Informativeness of human (dC-dA)n · (dG-Dt)n polymorphisms. Genomics 7: 524–530 (1990).
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Thomas, M.R., Cain, P. & Scott, N.S. DNA typing of grapevines: A universal methodology and database for describing cultivars and evaluating genetic relatedness. Plant Mol Biol 25, 939–949 (1994). https://doi.org/10.1007/BF00014668
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DOI: https://doi.org/10.1007/BF00014668