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A PCR-based method for the detection of genetically modified potatoes by the gene ac2 from Amaranthus caudatus

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Abstract

Genetically modified organisms have become a part of our everyday life. New modifications arise every year. The most of papers is focused on publication of detection protocols for genetically modified corn, soyabean, rape, or cotton. Minor modification, such as in potatoes attracts little attention. This work is based on developing an easy and cheap PCR method for the detection of an antimicrobial peptide from amaranthus which was used for transformation of potatoes. The base of the method is duplex PCR which enables identification of both potato and amaranthus specific genes in one tube. The method is enhanced by using positive plasmid controls which are able to reveal presence of inhibitors, reduce the incidence of false negative results, and wrong interpretation during analysis of routine samples.

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Acknowledgements

The work was partially supported by the grant No. MZE0002716201 from Ministry of Agriculture of the Czech Republic. Authors thank to Mgr. Maria Vass (Veterinary Research Institute) for critical reading of the manuscript, to Dr. Rozsypal (Research Institute of Crop Production, Prague, Czech Republic) for potatoes species mentioned in this work and to Dr. Zraly (Veterinary Research Institute, Brno, Czech Republic) for seeds of Amaranthus caudatus.

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Authors and Affiliations

  1. Veterinary Research Institute, Department of Food and Feed Safety, Hudcova 70, 621 32, Brno, Czech Republic

    Radka Pribylova, Ivo Pavlik, Zdenka Rozsypalova & Milan Bartos

Authors
  1. Radka Pribylova
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  2. Ivo Pavlik
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  3. Zdenka Rozsypalova
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  4. Milan Bartos
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Correspondence to Milan Bartos.

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Pribylova, R., Pavlik, I., Rozsypalova, Z. et al. A PCR-based method for the detection of genetically modified potatoes by the gene ac2 from Amaranthus caudatus . Eur Food Res Technol 223, 139–142 (2006). https://doi.org/10.1007/s00217-005-0171-2

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  • DOI: https://doi.org/10.1007/s00217-005-0171-2

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