Abstract
A cDNA clone encoding an antimicrobial chitin-binding protein from amaranth (Amaranthus caudatus L.) was isolated using a cDNA library constructed from near-mature seed poly(A)+ mRNA. The deduced amino acid sequence of the cDNA clone encodes a predicted polypeptide of 86 amino acids. This polypeptide has three distinct domains: an amino-terminal putative signal peptide (25 amino acids), a domain corresponding to the mature protein (30 amino acids), and a carboxyl-terminal propeptide (31 amino acids) containing a putative N-glycosylation site. The encoded protein differs from all known members of the family of chitin-binding proteins. Transcripts of the expected size (650 bp) are present in developing seeds but not in roots, leaves or stressed leaves.
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De Bolle, M.F.C., David, K.M.M., Rees, S.B. et al. Cloning and characterization of a cDNA encoding an antimicrobial chitin-binding protein from amaranth,Amaranthus caudatus . Plant Mol Biol 22, 1187–1190 (1993). https://doi.org/10.1007/BF00028991
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DOI: https://doi.org/10.1007/BF00028991