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Manipulate and stretch single pectin molecules with modified molecular combing and fluid fixation techniques

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Abstract

Manipulating and stretching single pectin molecules offer convenience to determine the chain lengths and further illustrate the action between pectins and other macromolecules. Pectin molecules on mica surfaces, which were imaged in air with atomic force microscopy (AFM), can be aligned and stretched through applying modified molecular combing and fluid fixation techniques. Modified molecular combing showed more effective and powerful for manipulation than fluid fixation. Chelate-soluble pectin (CSP) can be straightened into line to one direction by molecular combing while sodium carbonate-soluble pectin (SSP) shows ‘V’ structures. The differences may be the result of the different parts of the pectins which touched the mica surface firstly during the dropping, and the lengths of the chains. CSP was relatively long and initially touched the mica with the end point while SSP was short and touched the mica firstly with its middle part.

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Acknowledgements

This research was supported by grants from the National Key Technologies R and D Programme (Project No. 2004BA527B) and the National Economy and Commerce Committee (Project No. 02CJ-12-07-03).

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Authors and Affiliations

  1. Department of Food Science and Technology, Shanghai Jiao Tong University, 2678 Qixin Road, Shanghai, 201101, P.R. China

    Hongshun Yang & Yunfei Li

  2. Institute of Refrigeration and Cryogenics Engineering, Shanghai Jiao Tong University, Shanghai, 200030, P.R. China

    Hongshun Yang

  3. Nanobiology Laboratory, Shanghai Jiao Tong University, Shanghai, 200030, P.R. China

    Hongjie An

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  1. Hongshun Yang
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  2. Hongjie An
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  3. Yunfei Li
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Correspondence to Yunfei Li.

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Yang, H., An, H. & Li, Y. Manipulate and stretch single pectin molecules with modified molecular combing and fluid fixation techniques. Eur Food Res Technol 223, 78–82 (2006). https://doi.org/10.1007/s00217-005-0138-3

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  • DOI: https://doi.org/10.1007/s00217-005-0138-3

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