Abstract
A mixed immunosorbent, composed of anti-isoproturon and anti-chlortoluron antibodies immobilized on aldehyde-activated silica was evaluated for the trace enrichment and clean-up of several phenylurea herbicides from potatoes, carrots and peas. As the immunosorbent exhibited non-specific interactions with the matrix components, a previous clean-up step based on gel filtration (Sephadex G-25 PD-10 column) or anion-exchange (Power 1 × 8 Dionex column) was necessary. The coupling of gel filtration and immunosorbent with subsequent HPLC/UV determination allowed the determination of selected herbicides in potatoes in the 30 ng/g range with high precision, but for more complex samples (carrots and peas) the combination of anion-exchange resin and immunosorbent was required. With 30 ng/g spiked samples recovery rates for isoproturon, chlortoluron, metobromuron, linuron and chlorbromuron averaged 80 ± 20% (n = 5 in two consecutive days) for the whole procedure and the estimated detection limits ranged from 5 to 20 ng/g.
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Received: 29 January 1998 / Revised: 4 May 1998 / Accepted: 30 May 1998
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Herrera, S., Martín-Esteban, A., Fernández, P. et al. Evaluation of a mixed immunosorbent for selective trace enrichment of phenylurea herbicides from plant material. Fresenius J Anal Chem 362, 547–551 (1998). https://doi.org/10.1007/s002160051122
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DOI: https://doi.org/10.1007/s002160051122