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An Enzyme-Linked Immunosorbent Assay to Detect Salinomycin Residues Based on Immunomagnetic Bead Clean-up

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Abstract

Polyether antibiotics have been widely used for the treatment and prevention of coccidiosis in chicken farming. In the present study, an efficient, simple and inexpensive competitive indirect enzyme-linked immunosorbent assay (ciELISA) method based on immunomagnetic sample clean up was developed for salinomycin. Monoclonal antibodies were immobilized on the surface of carboxylic acid magnetic beads (2.8 μm in diameter). After a simple extraction, residues in sample extracts were specifically adsorbed and the supernatant removed by magnetic separation. Analytes retained on the beads were then released by elution prior to ciELISA. The limit of detection for salinomycin in chicken muscle and liver was 22 and 18 ng mL−1, respectively, and the linear quantitative range was 47–653 ng mL−1. Intra-assay recoveries ranged from 86.00 to 99.32%, and inter-assay recoveries were between 85.68 and 96.34%. The inhibition efficiency and sensitivity of this method was improved compared with traditional hydrophilic-lipophilic balance column clean up ciELISA. Furthermore, the convenience and repeatability of the immunomagnetic cleanup renders the new method of high value for the analysis of drug residues in complex matrices.

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Authors and Affiliations

  1. National Reference Laboratory of Veterinary Drug Residues, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, People’s Republic of China

    Wenhao Tian, Xiaoxiao Zhang, Meirong Song, Haiyang Jiang, Shuangyang Ding, Jianzhong Shen & Jiancheng Li

  2. Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, Beijing, 100193, People’s Republic of China

    Haiyang Jiang, Shuangyang Ding, Jianzhong Shen & Jiancheng Li

Authors
  1. Wenhao Tian
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  2. Xiaoxiao Zhang
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  3. Meirong Song
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  4. Haiyang Jiang
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  5. Shuangyang Ding
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  6. Jianzhong Shen
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  7. Jiancheng Li
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Correspondence to Jiancheng Li.

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Funding

This study was funded by National ‘Twelfth Five-Year’ Plan for Science and Technology Support (grant number 2011BAK10B06) and the National Natural Science Foundation of China (grant number 31272604).

Conflict of Interest

Wenhao Tian declares that he has no conflict of interest. Xiaoxiao Zhang declares that she has no conflict of interest. Meirong Song declares that he has no conflict of interest. Haiyang Jiang declares that she has no conflict of interest. Shuangyang Ding declares that she has no conflict of interest. Jianzhong Shen declares that he has no conflict of interest. Jiancheng Li declares that he has no conflict of interest.

Ethical Approval

All applicable China agricultural university laboratory animal management committee guidelines for the care and use of animals were followed.

This article does not contain any studies with human participants performed by any of the authors.

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Tian, W., Zhang, X., Song, M. et al. An Enzyme-Linked Immunosorbent Assay to Detect Salinomycin Residues Based on Immunomagnetic Bead Clean-up. Food Anal. Methods 10, 3042–3051 (2017). https://doi.org/10.1007/s12161-017-0873-7

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  • DOI: https://doi.org/10.1007/s12161-017-0873-7

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