Abstract
A highly sensitive fluorimetric method for the determination of fluoride was established. The method was based on quenching of the fluorescence of the Al3+-calcein complex in CCl3COOH-CH3COOK buffer solution by fluoride. The fluorescence emission was measured at λex/λem 480/503 nm, and the experimental variables and interference in this determination were studied. The linear calibration range was 0.8 ng/mL to 150 ng/mL and the detection limit was 0.2 ng/mL. The method was applied to determine fluoride in biological materials. The recovery was in the range of 96.3% to 104.7% and the relative standard deviation was 4.6%. The results obtained from the certified reference material by the present method agreed with the certified values.
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Received: 6 March 2000 / Revised: 26 April 2000 / Accepted: 29 April 2000
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Li, HB., Chen, F. A highly sensitive fluorimetric method for the determination of fluoride in biological material with Al3+-calcein complex. Fresenius J Anal Chem 368, 501–504 (2000). https://doi.org/10.1007/s002160000478
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DOI: https://doi.org/10.1007/s002160000478