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Trends in single-impact electrochemistry for bacteria analysis

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Abstract

Single-impact electrochemistry for the analysis of bacteria is a powerful technique for biosensing applications at the single-cell scale. The sensitivity of this electro-analytical method has been widely demonstrated based on chronoamperometric measurements at an ultramicroelectrode polarized at the appropriate potential of redox species in solution. Furthermore, the most recent studies display a continuous improvement in the ability of this sensitive electrochemical method to identify different bacterial strains with better selectivity. To achieve this, several strategies, such as the presence of a redox mediator, have been investigated for detecting and identifying the bacterial cell through its own electrochemical behavior. Both the blocking electrochemical impacts method and electrochemical collisions of single bacteria with a redox mediator are reported in this review and discussed through relevant examples. An original sensing strategy for virulence factors originating from pathogenic bacteria is also presented, based on a recent proof of concept dealing with redox liposome single-impact electrochemistry. The limitations, applications, perspectives, and challenges of single-impact electrochemistry for bacteria analysis are briefly discussed, based on the most significant published data.

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Acknowledgements

This work is supported by Nantes Université and Région Pays de la Loire (Rising stars program, e-NANOBIO).

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Authors and Affiliations

  1. Nantes Université, CNRS, CEISAM, UMR 6230, F-44000, Nantes, France

    Hassiba Smida, Arthur Langlard, Dorine Ameline, Christine Thobie-Gautier, Mohammed Boujtita & Estelle Lebègue

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  1. Hassiba Smida
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  2. Arthur Langlard
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  3. Dorine Ameline
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  4. Christine Thobie-Gautier
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  5. Mohammed Boujtita
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Correspondence to Estelle Lebègue.

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Published in the topical collection Young Investigators in (Bio-)Analytical Chemistry 2023 with guest editors Zhi-Yuan Gu, Beatriz Jurado-Sánchez, Thomas H. Linz, Leandro Wang Hantao, Nongnoot Wongkaew, and Peng Wu.

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Smida, H., Langlard, A., Ameline, D. et al. Trends in single-impact electrochemistry for bacteria analysis. Anal Bioanal Chem 415, 3717–3725 (2023). https://doi.org/10.1007/s00216-023-04568-z

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