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Certification of visinin-like protein-1 (VILIP-1) certified reference material by amino acid-based and sulfur-based liquid chromatography isotope dilution mass spectrometry

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Abstract

As an emerging neurodegenerative disease, Alzheimer’s disease (AD) has become a leading cause of dementia in older adults. Visinin-like protein-1 (VILIP-1) is an increasingly used biomarker for AD besides the widely accepted Aβ1-40, Aβ1-42, and tau. However, significant variations exist in the commercial immuno-based assays for VILIP-1 quantification, underlining the necessity to establish a traceability chain. Certified reference materials (CRMs) located at the top of the traceability chain are traceability sources for relevant matrix standard materials. In this work, VILIP-1 solution CRM with a certified value and uncertainty of 39.82±1.52 μg·g−1 was developed and certified using amino acid-based isotope dilution mass spectrometry (AA-ID-MS) and sulfur-based isotope dilution inductively coupled plasma mass spectrometry (ID-ICP-MS). Certified values from both strategies showed great consistency, with traceability to SI units. Moreover, the candidate VILIP-1 CRM shows excellent homogeneity and can be stable for at least 7 days at −20°C and 12 months at −70°C. The VILIP-1 CRM developed can be used in value assignment to secondary calibrators and clinical matrix CRMs, showing prospects in early diagnosis and disease monitoring for AD.

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Acknowledgements

The authors would like to thank Ming Li, Peng Xiao, Liqing Wu, and Hui Jiao in NIM for their assistance and support with the current work.

Funding

The work was supported by the National Natural Science Foundation of China (No. 12075230) and the National Key Research and Development Program of China (No. 2017YFF0205402, No. 2021YFC2103900).

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Authors and Affiliations

  1. Division of Chemical Metrology and Analytical Science, National Institute of Metrology, Beijing, 100029, People’s Republic of China

    Yang Zang, Xirui Zhou, Mengyun Pan, Yanli Lu, Hangrui Liu & Liuxing Feng

  2. College of Material Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China

    Yang Zang, Yanli Lu & Jinping Xiong

  3. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China

    Hangrui Liu

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  1. Yang Zang
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Correspondence to Xirui Zhou or Liuxing Feng.

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Zang, Y., Zhou, X., Pan, M. et al. Certification of visinin-like protein-1 (VILIP-1) certified reference material by amino acid-based and sulfur-based liquid chromatography isotope dilution mass spectrometry. Anal Bioanal Chem 415, 211–220 (2023). https://doi.org/10.1007/s00216-022-04401-z

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  • DOI: https://doi.org/10.1007/s00216-022-04401-z

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