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Colorimetric detection of Salmonella typhimurium based on hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles

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Abstract

We developed an effective and specific colorimetric strategy to detect Salmonella typhimurium (S. typhimurium) based on hexadecyl trimethyl ammonium bromide (CTAB)-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles (β-CD-AuNPs). In this study, ssDNA aptamer of S. typhimurium could combine with CTAB to form the supramolecular ssDNA-CTAB composite, so the ssDNA aptamer was applied to control the concentration of CTAB. In the presence of S. typhimurium, ssDNA aptamers selectively bound to S. typhimurium but not to CTAB, leading to the host–guest chemistry reaction of CTAB and β-CD resulting in β-CD-AuNP supramolecular assembly aggregation with an obvious color change. The ratio of absorption at 650 and 520 nm (A650nm/A520nm) has a linear correlation to the log scale of the concentration of the bacteria (1 × 102–1 × 107 CFU/mL) with a low limit of detection (LOD) of 13 CFU/mL. In addition, this optical sensor has good selectivity and practicability. In milk samples, the recovery was 93.55–111.32%, which suggested its potential application in real samples.

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Funding

This study was funded by the National Natural Science Foundation of China (Grant No. 82073557), Jilin Province Science and Technology Development Plan Item (Grant No. 20200602010ZP), and Interdisciplinary Research Funding Program for Doctoral Postgraduates of Jilin University (Grant No. 101832020DJX082).

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Authors and Affiliations

  1. School of Public Health, Jilin University, Changchun, 130021, China

    Shengnan Wei, Xuechen Wang, Hang Li, Zhenyue Su, Yuanyuan Guo, Xuening Shi, Juan Li & Chao Zhao

  2. Hospital of Stomatology, Jilin University, Changchun, 130021, China

    Feng Wang & Xinqing Hao

  3. The Department of Cadre Ward, The First Hospital of Jilin University, Changchun, 130021, China

    Xingxing Liu

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  1. Shengnan Wei
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  2. Xuechen Wang
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Correspondence to Xingxing Liu, Juan Li or Chao Zhao.

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Wei, S., Wang, X., Wang, F. et al. Colorimetric detection of Salmonella typhimurium based on hexadecyl trimethyl ammonium bromide-induced supramolecular assembly of β-cyclodextrin-capped gold nanoparticles. Anal Bioanal Chem 414, 6069–6076 (2022). https://doi.org/10.1007/s00216-022-04166-5

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