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Aptamer functionalized and reduced graphene oxide hybridized porous polymers SPE coupled with LC–MS for adsorption and detection of human α-thrombin

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Abstract

In this study, reduced graphene oxide (rGO) hybridized high internal phase emulsions were developed and polymerized as porous carriers for aptamer (5’/5AmMC6/-AGT CCG TGG TAG GGC AGG TTG GGG TGA CT-3’) modification to enrich human α-thrombin from serum. The structure and properties of the materials were confirmed by scanning electron microscope (SEM), Fourier transform infrared spectroscope (FT-IR), and X-ray photoelectron spectra (XPS). The adsorption ability and selectivity were studied and the thrombin was detected with liquid chromatography-mass spectrometry (LC–MS). The adsorption of thrombin onto the sorbent was achieved within 30 min and the desorption was realized using 5.0 mL of acetonitrile/water (80/20, v/v). The thrombin was quantified by LC–MS according to its characteristic peptide sequence of ELLESYIDGR.

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Funding

This research was supported by the National Natural Science Foundation of China (No. 21665006); the Natural Science Foundation from Guangxi Zhuang Autonomous Region (No. 2020GXNSFDA297025), the project of improving the basic scientific research ability of young and middle-aged teachers in Guangxi Universities (No. 2020KY12004), and Guangxi science and technology base and talent special project (No. 2019AC20330), respectively.

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Author notes

  1. Wenjuan Zhang and Haoyun Hu contributed equally to this work.

Authors and Affiliations

  1. Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, Guangxi, China

    Wenjuan Zhang, Haoyun Hu, Guihua Ruan, Yipeng Huang & Fuyou Du

  2. Geology & Mineral Analysis & Test Research Center of Guangxi Autonomous Region, Nanning, 530023, Guangxi, China

    Wenjuan Zhang

  3. College of Biological and Environmental Engineering, Changsha University, Changsha, 410003, China

    Fuyou Du

  4. Pharmacy School, Guilin Medical University, Guilin, 541004, Guangxi, China

    Zhengyi Chen

Authors
  1. Wenjuan Zhang
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  2. Haoyun Hu
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  3. Guihua Ruan
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  4. Yipeng Huang
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  5. Fuyou Du
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Correspondence to Guihua Ruan or Zhengyi Chen.

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Zhang, W., Hu, H., Ruan, G. et al. Aptamer functionalized and reduced graphene oxide hybridized porous polymers SPE coupled with LC–MS for adsorption and detection of human α-thrombin. Anal Bioanal Chem 414, 1553–1561 (2022). https://doi.org/10.1007/s00216-021-03776-9

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  • DOI: https://doi.org/10.1007/s00216-021-03776-9

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