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VS2 quantum dot label-free fluorescent probe for sensitive and selective detection of ALP

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Abstract

As an essential enzyme highly associated with various human diseases, alkaline phosphatase (ALP) plays an important role in human tissues. Developing new materials and strategies for monitoring ALP is thus important. We have developed a novel label-free fluorescent sensing system for ALP activity that is based on the “turn-on” fluorescence of VS2 quantum dots. The fluorescence of VS2 quantum dots quenched by Fe3+ can be restored by ascorbic acid, which is generated by hydrolysis of l-ascorbic acid 2-phosphate catalyzed by ALP. Rapid, convenient, and sensitive detection of ALP is achieved in the range from 3 to 1000 U/L (R 2 =0.9985), with a detection limit of 0.27 U/L. The proposed sensor exhibits excellent selectivity for ALP compared with other enzymes and proteins, such as glucose oxidase, lysozyme, trypsin, human serum albumin, and bovine serum albumin. The reliability for ALP determination in human serum plasma has been demonstrated with satisfactory recovery, revealing promising application in clinical diagnosis and biomedical research.

Hydrothermally synthesized VS2 quantum dots serving as a novel turn-on fluorescent probe for detection of alkaline phosphatase (ALP) activity. AA l-ascorbic acid, AAP l-ascorbic acid 2-phosphate, NAC N-acetyl-l-cysteine

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (no. 21475051).

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Authors and Affiliations

  1. Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, Jilin, 130012, China

    Xiaohan Ma, Cuicui Du, Mengxiang Shang & Wenbo Song

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  1. Xiaohan Ma
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  2. Cuicui Du
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  3. Mengxiang Shang
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  4. Wenbo Song
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Correspondence to Wenbo Song.

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The authors declare that they have no competing interests.

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This study was conducted in accordance with the principles of the Declaration of Helsinki, and was approved by the Ethical Committee of the First Hospital of Jilin University. All blood samples were from healthy persons with their informed consent.

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No violation of human or animal rights occurred during this investigation.

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Ma, X., Du, C., Shang, M. et al. VS2 quantum dot label-free fluorescent probe for sensitive and selective detection of ALP. Anal Bioanal Chem 410, 1417–1426 (2018). https://doi.org/10.1007/s00216-017-0778-8

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  • DOI: https://doi.org/10.1007/s00216-017-0778-8

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